Glutathione metabolism is essential for self-renewal and chemoresistance of pancreatic cancer stem cells

Table 1 List of utilized primers

Gene	Primer
HPRT
Forward	TGACCTTGATTTATTTTGCATACC
Reverse	CGAGCAAGACGTTCAGTCCT
GCLC
Forward	GCCGCTGAGCTGGGAGGAAA
Reverse	ATTCCACCTCATCGCCCCACT
GPX1
Forward	TATCGAGAATGTGGCGTCCC
Reverse	GATGCCCAAACTGGTTGCAC
GPX2
Forward	GGACATCAGGAGAACTGTCAGA
Reverse	TCAGGTAGGCGAAGACAGGA
GGT1
Forward	CAACCTGCCCACAGTGAAGA
Reverse	TTCTTGGCCTCCATGACTGC
GGT2
Forward	CCCTTCTTTCAGTGGGAGGG
Reverse	ATGCTGTCACCTTTGTGGCT
GSTM1
Forward	AGGAAAAGAAGTACACGATGGG
Reverse	TTGCTCTGGGTGATCTTGTG
GSTA1
Forward	CAACCTGCCCACAGTGAAGA
Reverse	TTCTTGGCCTCCATGACTGC
GSTA2
Forward	CCCTTCTTTCAGTGGGAGGG
Reverse	ATGCTGTCACCTTTGTGGCT
GSTA
Forward	TCCGTGAGATGGGTTTTAGC
Reverse	CTGTACCAACTTCATCCCGTC
IDH1
Forward	CAAGTGACGGAACCCAAAAG
Reverse	ACCCTTAGACAGAGCCATTTG
IDH2
Forward	ACAACACCGACGAGTCCATC
Reverse	GCCCATCGTAGGCTTTCAGT

Table 2 RNAseq reveals that glutathione metabolism genes are up-regulated in cancer stem cell-enriched cultures from pancreatic cancer patient-derived xenografts

PDX	GGTs	GSTs	GPXs	Synthesis and recycling
PDX185	GGT1, GGT2, GGT3P, GGT6, GGT7	GSTA1, GSTA2, GSTT1, MGST2, MGST3	GPX2, GPX3	GCLC, GSR, PGD, IDH1
PDX215	GGT1, GGT2, GGT7, GGT8P	GSTA1, GSTA4, GSTM2, GSTM4, MGST2		OPLAH, IDH1
PDX253	GGT1, GGT2, GGT3P, GGT6	GSTA1, GSTA2, GSTA4, MGST1, MGST2, MGST3	GPX2, GPX8	GSR, IDH1
PDX354		GSTA1, GSTA2, MGST1, GSTM2	GPX3, GPX8	GCLC, GGCT, IDH1
PDX265		GSTA4, GSTM1, GSTM2, GSTM4	GPX2	GGCT, PGD, IDH1, IDH2

PDX: Patient-derived xenograft; GGTs: Gamma-glutamyltransferases; GSTs: Glutathione-s-transferases; GPXs: Glutathione peroxidases.