Anti-osteoarthritis effect of a combination treatment with human adipose tissue-derived mesenchymal stem cells and thrombospondin 2 in rabbits

doi:10.4252/wjsc.v11.i12.1115

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Dec 26, 2019; 11(12): 1115-1129
Published online Dec 26, 2019. doi: 10.4252/wjsc.v11.i12.1115

Table 1 Radiological evaluation scores 8 wk after cell transplantation

Treatment (dose)	Osteophytes in medial femoral condyle (0-5)	Deformity of medial tibia (0-5)	Sharpening of tibial spine (0-5)	Total score (0-15)
ACLT alone	3.5 ± 0.2	3.2 ± 0.4	2.7 ± 0.3	9.3 ± 0.6
hADMSCs (low)	3.0 ± 0.4	3.0 ± 0.4	2.7 ± 0.2	8.7 ± 0.4
hADMSCs (high)	2.7 ± 0.2	2.3 ± 0.2	2.2 ± 0.2	7.2 ± 0.3¹
hADMSCs (low) + TSP2	2.3 ± 0.3	1.5 ± 0.3	2.5 ± 0.3	6.0 ± 0.4¹
TSP2 (100 ng)	2.5 ± 0.3	2.5 ± 0.3	2.3 ± 0.3	7.2 ± 0.8¹

¹Significantly different from ACLT alone, P < 0.05. ACLT: Anterior cruciate ligament transection; hADMSCs: Human adipose-derived mesenchymal stem cells; TSP2: Thrombospondin 2.

Table 2 Gross evaluation scores

Treatment (dose)	Roughness of surface	Adhesion with meniscus	Hypertrophy of joint	Osteophyte	Total score
Treatment (dose)	(0-8)	(0-8)	(0-8)	(0-8)	(0-32)
ACLT alone	5.5 ± 0.5	4.5 ± 0.5	6.0 ± 0.4	4.0 ± 2.0	20.0 ± 3.1
hADMSCs (low)	4.0 ± 1.0	4.0 ± 1.0	5.0 ± 1.0	4.0 ± 1.2	17.0 ± 2.2
hADMSCs (high)	2.0 ± 1.1	2.5 ± 0.5	2.5 ± 1.5	1.5 ± 0.5	8.5 ± 2.5¹
hADMSCs (low) + TSP2	3.0 ± 0.2	1.5 ± 0.6	1.0 ± 0.4	2.0 ± 0.6	7.5 ± 0.5¹
TSP2 (100 ng)	1.5 ± 1.5	3.5 ± 0.5	3.5 ± 1.5	3.0 ± 1.1	11.5 ± 4.5¹

¹Significantly different from ACLT alone, P < 0.05. ACLT: Anterior cruciate ligament transection; hADMSCs: Human adipose-derived mesenchymal stem cells; TSP2: Thrombospondin 2.

Table 3 Microscopic evaluation scores

Treatment (dose)	Structure (0-11)	Cellularity (0-4)	Cluster (0-3)	Proteoglycan (0-6)	Collagen II (0-6)	Total score (0-30)
ACLT alone	7.5 ± 1.5	3.0 ± 1.1	2.0 ± 1.0	6.0 ± 0.3	4.5 ± 1.5	23.0 ± 5.0
hADMSCs (low)	8.0 ± 0.5	1.0 ± 1.0	1.5 ± 1.5	4.5 ± 0.5	3.0 ± 0.4	18.0 ± 2.0
hADMSCs (high)	2.5 ± 1.5	2.0 ± 1.0	0.0 ± 0.0	2.5 ± 0.4	1.5 ± 0.5	8.5 ± 3.5¹
hADMSCs (low) + TSP2	1.0 ± 0.2	3.0 ± 0.5	0.0 ± 0.0	2.0 ± 0.3	1.5 ± 0.5	7.5 ± 0.6¹
TSP2 (100 ng)	1.5 ± 0.5	2.0 ± 0.2	1.5 ± 1.5	4.0 ± 0.7	1.5 ± 1.5	10.5 ± 2.5¹

¹Significantly different from ACLT alone, P < 0.05. ACLT: Anterior cruciate ligament transection; hADMSCs: Human adipose-derived mesenchymal stem cells; TSP2: Thrombospondin 2.

Citation: Shin K, Cha Y, Ban YH, Seo DW, Choi EK, Park D, Kang SK, Ra JC, Kim YB. Anti-osteoarthritis effect of a combination treatment with human adipose tissue-derived mesenchymal stem cells and thrombospondin 2 in rabbits. World J Stem Cells 2019; 11(12): 1115-1129
URL: https://www.wjgnet.com/1948-0210/full/v11/i12/1115.htm
DOI: https://dx.doi.org/10.4252/wjsc.v11.i12.1115