Basic Study
Copyright ©The Author(s) 2023.
World J Stem Cells. Sep 26, 2023; 15(9): 897-907
Published online Sep 26, 2023. doi: 10.4252/wjsc.v15.i9.897
Figure 1
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Figure 1 Morphology of adipose-derived stem cells at passage 4 (× 100).
Figure 2
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Figure 2 Detection of cell surface markers by flow cytometry. A: Positive expression of CD44; B: Positive expression of CD44; C: Negative expression of CD45; D: Negative expression of CD34.
Figure 3
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Figure 3 Morphology and protein identification of adipose-derived stem cell-derived exosomes. A: Adipose-derived stem cell (ADSC)-derived exosome morphology seen using transmission electron microscopy (× 15000); B: Surface detection of ADSC-derived exosomes by western blotting. ADSC: Adipose-derived stem cell.
Figure 4
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Figure 4 Hematoxylin and eosin staining of rat myocardial tissue. A: Myocardial tissue of rats in control group; B: Myocardial tissue of rats in heart failure group; C: Myocardial tissue of rats in adipose-derived stem cell-exosome group; Col: Control; HF: Heart failure; ADSCs-Exo: Adipose-derived stem cell-exosome.
Figure 5
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Figure 5 Comparison of cardiomyocyte apoptosis rate in each group. A: Cardiomyocyte apoptosis rate in control group; B: Cardiomyocyte apoptosis rate in heart failure group; C: Cardiomyocyte apoptosis rate in adipose-derived stem cell-exosome group. Col: Control; HF: Heart failure; ADSCs-Exo: Adipose-derived stem cell-exosome.
Figure 6
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Figure 6 Comparison of ATP content in myocardial tissue of rats in each group. A: Apoptosis rate of cardiomyocytes in three groups of rats; B: ATP content in myocardial tissue of rats in three groups. aP < 0.05 compared with the control group; bP < 0.05 compared with the heart failure group. Col: Control; HF: Heart failure; ADSCs-Exo: Adipose-derived stem cell-exosome.
Figure 7
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Figure 7 Comparison of the protein expression levels of Bcl-2, Bax, caspase-3, and p53 in the myocardial tissue of rats in each group. A: Myocardial tissue protein electrophoresis detection; B: Protein expression levels of Bcl-2; C: Protein expression levels of Bax; D: Protein expression levels of caspase-3; E: Protein expression levels of p53. aP < 0.05 compared with the control group; bP < 0.05 compared with the heart failure group. Col: Control; HF: Heart failure; ADSCs-Exo: Adipose-derived stem cell-exosome.