Wang FD, Ding Y, Zhou JH, Zhou E, Zhang TT, Fan YQ, He Q, Zhang ZQ, Mao CY, Zhang JF, Zhou J. Gamma-aminobutyric acid enhances miR-21-5p loading into adipose-derived stem cell extracellular vesicles to alleviate myocardial ischemia-reperfusion injury via TXNIP regulation. World J Stem Cells 2024; 16(10): 873-895 [PMID: 39493825 DOI: 10.4252/wjsc.v16.i10.873]
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03618516
Submitted on:
October 25, 2024, 09:55
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Reader Comments:
The original article by Wang FD et al., "Gamma-aminobutyric acid enhances miR-21-5p loading into adipose-derived stem cell extracellular vesicles to alleviate myocardial ischaemia-reperfusion injury via TXNIP regulation," is very good.
GABA-induced miR-21-5p-loaded EVs utilise a unique mechanism to lessen myocardial ischaemia-reperfusion injury (MIRI) and present a novel method for managing MIRI through oxidative stress modulation. The study reported that miR-21-5p exerts its effects through a direct interaction with TXNIP in cardiomyocytes, thereby triggering oxidative stress and elucidating related molecular mechanisms. Furthermore, this work systematically employs in vivo, in vitro, and a variety of imaging methods with gene knockout models to provide comprehensive methodological proofs.
Considering myocardial ischaemia is a common clinical condition, this study has high translational relevance.
But there are few queries:
The experiment only evaluates its EV source in inguinal ADSCs and could miss other potential beneficial configurations of EV.
It is still unclear whether these short-term therapeutic effects are sustainable or if they interfere with repeated dosing against MIRI, despite the study's lack of a long-term assessment.
The process of producing, standardizing, and ensuring the safety of EVs for human use could potentially complicate the clinical translation of the latter strategy.
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