Basic Research
Copyright ©The Author(s) 2003.
World J Gastroenterol. Aug 15, 2003; 9(8): 1767-1771
Published online Aug 15, 2003. doi: 10.3748/wjg.v9.i8.1767
Table 1 Primer sequences of iNOS, eNOS, COX-1, COX-2, HO-1, HO-2 and β-actin
PrimerPrimer sequencesAmplicon-length (bp)
HO-1Sense: 5’-CAGTCGCCTCCAGAGTTTCC-3’
Antisense: 5’-TACAAGGAGGCCATCACCAGC-3’284
HO-2Sense: 5’-AGAAGTATGTGGATCGGA-3’
Antisense: 5’-TACTCAGGTCCAAGGCA-3’242
COX-1Sense: 5’-TGCTGCTGAGAAGGGAGTTCATTC-3’
Antisense: 5’-CAAGTCACACACACGGTTATGCTC-3’403
COX-2Sense: 5’-ACACTCTATCACTGGCATCC-3’
Antisense: 5’-GAAGGGACACCCTTTCACAT-3’584
iNOSSense: 5’ -TGGCTTGCCCTTGGAAGTTTCTC-3’
Antisense: 5’-TCCAGGCCATCTTGGTGGCAAGA-3’574
eNOSSense: 5’-TACGGAGCAGCAAATCCAC-3’
Antisense: 5’-CAGGCTGCAGTCCTTTGATC-3’812
β-actinSense:5’-GTGGGGCGCCCCAGGCACCA-3’
Antisense: 5’-CTCCTTAATGTCACGCACGATTTC-3’540