Peptidergic innervation of human esophageal and cardiac carcinoma

Table 1 Antibodies of neuropeptides and their work dilution

Antibody	Working dilution
CGRP	1:8000
GAL	1:8000
SP	1:5000
NT	1:4000
SOM	1:8000
CCK	1:4000
L-ENK	1:5000
Dyn	1:4000
NPY	1:6000
M-ENK	1:6000

CGRP: Calcitonin gene-related peptide, GAL: Galanin, SP: Substance P, NT: Neurotensin, SOM: Somatostatin, CCK: Cholecystokinin, L-ENK: Leu-enkephalin, Dyn: Dynorphine, NPY:Neuropeptide Y, M-ENK: Met-enkephalin.

Table 2 Distribution of neuropeptide-immunoreactive nerve fibers in tumors

Antibodies	Cardiac cancer (n = 13)			Esophageal cancer (n = 16)
	+++	++	+	+++	++	+
CGRP	0	2	2	0	2	2
GAL	2	6	4	3	7	4
SP	1	5	1	2	5	5
NT	0	0	0	0	0	0
SOM	0	3	3	0	3	6
CCK	0	0	1	0	0	3
L-ENK	1	3	1	0	3	7
Dyn	2	3	5	0	8	1
NPY	2	5	3	1	6	6
M-ENK	3	3	2	0	5	7

Table 3 Outgrowth of processes of DRG co-cultured with tu-mor blocks

Tumor group	DRG with processes (n)	DRG without processes (n)	Rate of DRG with processes (x ± s)
Esophageal	49	28	0.63 ± 0.10a
Cardiac	60	29	0.67 ± 0.09a
Control	0	63	0

^aP < 0.0001 vs the control group