Experimental and clinic-opathologic study on the relationship between transcription factor Egr-1 and esophageal carcinoma

Table 1 Soft agar assays in Eca109 cell line

Cell line	No. of seeded cell	No. of colonies				CFR
		1	2	3	Mean
Eca109	1000	65	71	73	69 ± 2.4	6.9%
Egr-1-Eca109	1000	40	43	39	40 ± 1.2	4.0%b

^bP < 0.01, vs Eca109, U test.

Table 2 Tumorigenicity assays in Eca109 cell line

Cell line	Tumorigenicity rate	Growth rate of tumors (mg·d-1)
		1	2	3	4	5	6	Mean
Eca109	4/4	83.3	70.0	46.6	63.3			65.8 ± 7.6
Egr-1-Eca109	6/6	20.0	13.3	33.3	66.6	43.0	36.7	35.5 ± 7.6a

^aP < 0.05, vs Eca109, Student’s t test.

Table 3 Expression of Egr-1mRNA and proteins in various pathological changes of esophagus, n (%)

Group	n	ISH	IHC
Simple hyperplasia	30	12 (40.0)	9 (30.0)
Dysplasia	38	25 (65.8)	22 (57.9)a
Esophageal cancer	50	10 (20.0)	7 (14.0)b

^aP < 0.05, vs simple hyperplasia, χ² test;

^bP < 0.01, vs dysplasia, χ² test.