APC and K-ras gene mutation in aberrant crypt foci of human colon

Table 1 Primers for K-ras and APC gene sequencing

Genes	Region	Codon	Size of PCR	AT	Sequence
K-ras	exon1	1-54	163	56 °C	5-GACTGAATATAAACTTGTGG
					5-CTGTATCAAAGAAGTGTCCT
K-ras	exon2	31-84	161BP	54 °C	5-GACTGTGTTTCTCCCTTCT
					5-GGCAAATACACAAAGAAAG
APC	15-A	1263-1393	390BP	54 °C	5-GTGTAGAAGATACTCCAATA
					5-GTGAACTGACAGAAGTACAT
APC	15-B	1338-1436	295BP	56 °C	5-CAGGGTTCTAGTTTATCTTC
					5-TTCTGCTTGGTGGCATGGTT
APC	15-C	1412-1515	310BP	56 °C	5-GGAATGGTAAGTGGCATAAT
					5-AAATGGCTCATCGAGGCTCA
APC	15-D	1496-1596	300BP	56 °C	5-ACTCCAGATGGATTTTCTTG
					5-GGCTGGCTTTTTTGCTTTAC

Table 2 APC gene mutation

DNA	Region	Codon	Mutation	Amino acid exchange
N 3 CRC	A	1354	TTT→TTA	Phe→Leu
N17 CRC	A	1309	GAA→TAA	Stop cocon
N14 CRC	B	1389	TCT→TTT	Ser→Phe
N 5 CRC	B	1357	GGA→AGA	Gly→Arg
N21 CRC	B	1365	GGT→GGC	nonsense
N26 CRC	B	1398	AGT→ACT	Ser→Thr
N28 CRC	C	1465	GTG→GCG	Val→Ala
N33 CRC	D	1547	GAA→TAA	Stop codon
Tubular villous adenoma	A	1301	ins1 A	Stop codon
Villous adenoma	A	1309	GAA→TAA	Stop codon
Villous adenoma	C	1490	ins8 TTATTACA	Frame shift
Tubular villous adenoma	B	1367	CAG→CAC	Gln→His
ACF	A	1309	GAA→TAA	Stop codon