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Basic Study
Copyright: ©Author(s) 2026.
World J Gastroenterol. Apr 7, 2026; 32(13): 115299
Published online Apr 7, 2026. doi: 10.3748/wjg.v32.i13.115299
Table 1 The key reagent or resource
Key reagent or resource
Source
Identifier
Country
Anti-GSDMDAffinityAF4012United States
Anti-ASCAffinityDF6304United States
Anti-ZO-1Proteintech21773-1-APChina
Anti-claudin-1 Proteintech13050-1-APChina
Anti-β-actinAffinityAF7018United States
HRP-labeled goat anti-rabbit IgG (H + L)AffinityS0001United States
Dextran sulfate sodiumMP Biochemicals0216011090United States
DisulfiramMed Chem ExpressHY-B0240United States
BerberineYuanyeB21379China
TRIzol ThermoFisher15596018United States
Brilliant SYBR Green QPCR Master MixTransGenAQ211-01China
ELISA kit for mouse IL-1βElabscienceE-EL-M0037China
ELISA kit for mouse IL-6ElabscienceE-EL-M0044China
ELISA kit for mouse TNF-αElabscienceE-EL-M3063China
Chemiluminescence (ECL) substrateNCMP10300China
Protease inhibitor mixtureAladdinC129408China
Polyvinylidene fluoride membranesSigma-AldrichIPVH0010United States
Multiskan Skyhigh With Touch ScreenThermo Fisher ScientificA51119600United States
Chemiluminescence imaging systemShanghai Qinxing Scientific Instrument Co., LtdChemiScope 6200 TouchChina
MagPure Soil DNA LQ KitMagenD6356-02China
Western blot electrophoresis systemBio-Rad Laboratories552BRUnited States
Illumina sequencing adapteOE Biotech Co., LtdChina
NanoDrop 2000 spectrophotometerThermo Fisher ScientificUnited States
Real-time fluorescence quantitative PCR systemBioer Technology Co., LtdChina
Table 2 Sequences of primers used for reverse transcription quantitative polymerase chain reaction
Primer
Primer sequence (5’-3’)
Primer sequence (5’-3’)
IL-1βGCAACTGTTCCTGAACTCAACTATCTTTTGGGGTCCGTCAACT
IL-6CTTGGGACTGATGCTGGTGACGCCATTGCACAACTCTTTTCTC
TNF-αTACTGAACTTCGGGGTGATCGTCCTCCACTTGGTGGTTTGC
HprtGTCCCAGCGTCGTGATTAGCTGGCCTCCCATCTCCTTCA