Copyright: ©Author(s) 2026.
World J Gastroenterol. Apr 7, 2026; 32(13): 115299
Published online Apr 7, 2026. doi: 10.3748/wjg.v32.i13.115299
Published online Apr 7, 2026. doi: 10.3748/wjg.v32.i13.115299
Table 1 The key reagent or resource
| Key reagent or resource | Source | Identifier | Country |
| Anti-GSDMD | Affinity | AF4012 | United States |
| Anti-ASC | Affinity | DF6304 | United States |
| Anti-ZO-1 | Proteintech | 21773-1-AP | China |
| Anti-claudin-1 | Proteintech | 13050-1-AP | China |
| Anti-β-actin | Affinity | AF7018 | United States |
| HRP-labeled goat anti-rabbit IgG (H + L) | Affinity | S0001 | United States |
| Dextran sulfate sodium | MP Biochemicals | 0216011090 | United States |
| Disulfiram | Med Chem Express | HY-B0240 | United States |
| Berberine | Yuanye | B21379 | China |
| TRIzol | ThermoFisher | 15596018 | United States |
| Brilliant SYBR Green QPCR Master Mix | TransGen | AQ211-01 | China |
| ELISA kit for mouse IL-1β | Elabscience | E-EL-M0037 | China |
| ELISA kit for mouse IL-6 | Elabscience | E-EL-M0044 | China |
| ELISA kit for mouse TNF-α | Elabscience | E-EL-M3063 | China |
| Chemiluminescence (ECL) substrate | NCM | P10300 | China |
| Protease inhibitor mixture | Aladdin | C129408 | China |
| Polyvinylidene fluoride membranes | Sigma-Aldrich | IPVH0010 | United States |
| Multiskan Skyhigh With Touch Screen | Thermo Fisher Scientific | A51119600 | United States |
| Chemiluminescence imaging system | Shanghai Qinxing Scientific Instrument Co., Ltd | ChemiScope 6200 Touch | China |
| MagPure Soil DNA LQ Kit | Magen | D6356-02 | China |
| Western blot electrophoresis system | Bio-Rad Laboratories | 552BR | United States |
| Illumina sequencing adapte | OE Biotech Co., Ltd | China | |
| NanoDrop 2000 spectrophotometer | Thermo Fisher Scientific | United States | |
| Real-time fluorescence quantitative PCR system | Bioer Technology Co., Ltd | China |
Table 2 Sequences of primers used for reverse transcription quantitative polymerase chain reaction
| Primer | Primer sequence (5’-3’) | Primer sequence (5’-3’) |
| IL-1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT |
| IL-6 | CTTGGGACTGATGCTGGTGAC | GCCATTGCACAACTCTTTTCTC |
| TNF-α | TACTGAACTTCGGGGTGATCG | TCCTCCACTTGGTGGTTTGC |
| Hprt | GTCCCAGCGTCGTGATTAGC | TGGCCTCCCATCTCCTTCA |
- Citation: Yang P, Wang R, Zhou YP, Wen JF, Yang DX. Berberine alleviates experimental colitis by enhancing gut-microbiota-dependent intestinal barrier function and suppressing gasdermin D activation. World J Gastroenterol 2026; 32(13): 115299
- URL: https://www.wjgnet.com/1007-9327/full/v32/i13/115299.htm
- DOI: https://dx.doi.org/10.3748/wjg.v32.i13.115299
