Basic Study
Copyright ©The Author(s) 2025.
World J Gastroenterol. Jan 21, 2025; 31(3): 95207
Published online Jan 21, 2025. doi: 10.3748/wjg.v31.i3.95207
Table 1 miRNA-mRNA interactions observed in HepG2 and SNU449 cells treated with Sorafenib for 6 and 24 hours
Condition
hsa-miR
log2 (Fold-change)
P value
P-adj
mRNA target
Transcript ID
log2 (Fold-change)
P-adj
HepG2 6 hourshsa-miR-29b-3p-1.3743.23E-020.9961HRKENSG00000135116.91.9678.926E-08
HepG2 6 hourshsa-miR-29b-3p-1.3743.23E-020.9961JMYENSG00000152409.91.1052.253E-13
HepG2 6 hourshsa-miR-29b-3p-1.3743.23E-020.9961TNFAIP3ENSG00000118503.151.1864.131E-07
HepG2 6 hourshsa-miR-19b-3p-1.2774.29E-020.9961RORAENSG00000069667.161.0481.506E-03
HepG2 6 hourshsa-miR-19b-3p-1.2774.29E-020.9961TNFAIP3ENSG00000118503.151.1864.131E-07
HepG2 6 hourshsa-miR-19b-3p-1.2774.29E-020.9961ZNF385BENSG00000144331.201.1591.233E-02
HepG2 6 hourshsa-miR-19b-3p-1.2774.29E-020.9961ARRDC3ENSG00000113369.91.6974.636E-30
HepG2 24 hourshsa-miR-4521-4.4961.09E-040.1484MBNL1ENSG00000152601.171.2422.599E-16
HepG2 24 hourshsa-miR-7974-3.4321.97E-420.0000CREB3 L3ENSG00000060566.141.0624.001E-06
HepG2 24 hourshsa-miR-194-3p-1.4632.73E-080.0000ARHGAP24ENSG00000138639.181.2811.186E-08
HepG2 24 hourshsa-miR-194-3p-1.4634.47E-050.0020HOXA1ENSG00000105991.91.1922.484E-06
HepG2 24 hourshsa-miR-374a-3p-1.3274.47E-050.0020SH3GLB1ENSG00000097033.141.2961.800E-13
HepG2 24 hourshsa-miR-374a-3p-1.3278.95E-030.1859OXR1ENSG00000164830.181.0502.089E-08
HepG2 24 hourshsa-miR-374a-3p-1.3278.95E-030.1859GOLGA4ENSG00000144674.161.2308.419E-03
HepG2 24 hourshsa-miR-374a-3p-1.3278.95E-030.1859ARRDC3ENSG00000113369.92.8492.203E-41
HepG2 24 hourshsa-miR-27b-5p-1.2368.95E-030.1859OPTNENSG00000123240.171.5316.346E-20
HepG2 24 hourshsa-miR-193b-3p-1.1933.74E-040.0143SIAH1ENSG00000196470.121.5811.144E-38
HepG2 24 hourshsa-miR-193b-3p-1.1932.29E-020.3864LAMC2ENSG00000058085.151.3272.152E-03
HepG2 24 hourshsa-miR-3187-3p-1.1692.29E-020.3864PLK2ENSG00000145632.151.6343.009E-08
HepG2 24 hourshsa-miR-3187-3p-1.1693.59E-020.4538ATOH8ENSG00000168874.131.6916.502E-08
HepG2 24 hourshsa-miR-374b-3p-1.0643.59E-020.4538SMAD7ENSG00000101665.91.9991.669E-42
HepG2 24 hourshsa-miR-374b-3p-1.0647.47E-030.1693TIRARPENSG00000163659.131.0701.555E-18
HepG2 24 hourshsa-miR-769-5p-1.0587.47E-030.1693TFAP4ENSG00000090447.121.0101.161E-11
HepG2 24 hourshsa-miR-769-5p-1.0588.79E-030.1859FAXDC2ENSG00000170271.111.5651.249E-13
HepG2 24 hourshsa-miR-44889.0598.79E-030.1859MELTFENSG00000163975.12-1.0852.722E-05
SNU 6 hourshsa-miR-15a-5p-1.5269.20E-030.6536CHAC1ENSG00000128965.132.2625.787E-28
SNU 6 hourshsa-miR-12135-1.2982.11E-020.6536FOXJ1ENSG00000129654.81.2402.099E-06
SNU 24 hourshsa-miR-29b-1-5p-2.1262.50E-050.0007HEY1ENSG00000164683.171.6922.304E-07
SNU 24 hourshsa-miR-374c-5p-1.8845.25E-030.0787CEBPBENSG00000172216.61.0025.306E-17
SNU 24 hourshsa-miR-374c-5p-1.8845.25E-030.0787VEGFAENSG00000112715.221.4482.131E-45
SNU 24 hourshsa-miR-374c-5p-1.8845.25E-030.0787ADMENSG00000148926.102.3401.782E-143
SNU 24 hourshsa-miR-32-3p-1.8219.41E-040.0175YOD1ENSG00000180667.101.6581.063E-111
SNU 24 hourshsa-miR-32-3p-1.8219.41E-040.0175TNFAIP3ENSG00000118503.152.3163.471E-10
SNU 24 hourshsa-let-7b-3p-1.5411.72E-020.1785KLF4ENSG00000136826.151.5721.166E-31
SNU 24 hourshsa-let-7b-3p-1.5411.72E-020.1785ADRB2ENSG00000169252.51.7439.094E-48
SNU 24 hourshsa-miR-195-5p-1.4561.51E-020.1770VEGFAENSG00000112715.221.4482.131E-45
SNU 24 hourshsa-miR-195-5p-1.4561.51E-020.1770ADRB2ENSG00000169252.51.7439.094E-48
SNU 24 hourshsa-miR-34a-5p-1.3162.62E-020.2364KLF4ENSG00000136826.151.5721.166E-31
SNU 24 hourshsa-miR-34a-5p-1.3162.62E-020.2364HSPA1BENSG00000204388.71.6762.822E-82
SNU 24 hourshsa-miR-57011.5353.44E-020.2740SYNGR3ENSG00000127561.15-1.5051.627E-08
SNU 24 hourshsa-miR-57011.5353.44E-020.2740RFLNBENSG00000183688.4-1.3401.864E-27
SNU 24 hourshsa-miR-121363.3344.93E-160.0000ACVR2BENSG00000114739.14-1.0753.211E-13
SNU 24 hourshsa-miR-121363.3344.93E-160.0000NR4A2ENSG00000153234.14-1.7549.174E-06
SNU 24 hourshsa-miR-121363.3344.93E-160.0000CSPG5ENSG00000114646.10-1.0891.709E-08
SNU 24 hourshsa-miR-121363.3344.93E-160.0000AREGENSG00000109321.11-1.0162.623E-03
SNU 24 hourshsa-miR-121363.3344.93E-160.0000NRARPENSG00000198435.4-1.7633.743E-12
SNU 24 hourshsa-miR-121363.3344.93E-160.0000MAFENSG00000178573.7-1.7485.311E-04
SNU 24 hourshsa-miR-44928.4274.75E-120.0000IGFBP5ENSG00000115461.5-1.0732.379E-18
SNU 24 hourshsa-miR-448813.0911.08E-320.0000PDGFBENSG00000100311.17-1.1011.086E-51
Table 2 Predicted interactions between miRNAs and mRNAs, and between their corresponding lncRNAs in HepG2 and SNU449 cells treated with Sorafenib for 6 and 24 hours
Condition
miRNA
log2 (Fold-change)
Target
Target type
Transcript ID
log2 (Fold-change)
P-adj
HepG2 6 hourshsa-miR-19b-3p-1.277RORAmRNAENSG00000069667.161.0481.51E-03
HepG2 6 hourshsa-miR-19b-3p-1.277TNFAIP3mRNAENSG00000118503.151.1864.13E-07
HepG2 6 hourshsa-miR-19b-3p-1.277ZNF385BmRNAENSG00000144331.201.1591.23E-02
HepG2 6 hourshsa-miR-29b-3p-1.374HRKmRNAENSG00000135116.91.9678.93E-08
HepG2 6 hourshsa-miR-29b-3p-1.374JMYmRNAENSG00000152409.91.1052.25E-13
HepG2 6 hourshsa-miR-29b-3p-1.374TNFAIP3mRNAENSG00000118503.151.1864.13E-07
HepG2 24 hourshsa-miR-193b-3p-1.193CYTORlncRNAENSG00000222041.111.4217.45E-21
HepG2 24 hourshsa-miR-193b-3p-1.193MIR4435-2HGlncRNAENSG00000172965.161.2047.19E-10
HepG2 24 hourshsa-miR-193b-3p-1.193PCBP2-OT1lncRNAENSG00000282977.11.0868.35E-03
HepG2 24 hourshsa-miR-193b-3p-1.193ZFAS1lncRNAENSG00000177410.131.4134.96E-35
HepG2 24 hourshsa-miR-193b-3p-1.193SIAH1mRNAENSG00000196470.121.5811.14E-38
HepG2 24 hourshsa-miR-193b-3p-1.193LAMC2mRNAENSG00000058085.151.3272.15E-03
HepG2 24 hourshsa-miR-3187-3p-1.169MIR4435-2HGlncRNAENSG00000172965.161.2047.19E-10
HepG2 24 hourshsa-miR-3187-3p-1.169DANT2lncRNAENSG00000235244.41.0141.37E-02
HepG2 24 hourshsa-miR-3187-3p-1.169PLK2mRNAENSG00000145632.151.6343.01E-08
HepG2 24 hourshsa-miR-3187-3p-1.169ATOH8mRNAENSG00000168874.131.6916.50E-08
HepG2 24 hourshsa-miR-374a-3p-1.327MIR4435-2HGlncRNAENSG00000172965.161.2047.19E-10
HepG2 24 hourshsa-miR-374a-3p-1.327OXR1mRNAENSG00000164830.181.0502.09E-08
HepG2 24 hourshsa-miR-374a-3p-1.327GOLGA4mRNAENSG00000144674.161.2308.42E-03
HepG2 24 hourshsa-miR-374a-3p-1.327ARRDC3mRNAENSG00000113369.92.8492.20E-41
HepG2 24 hourshsa-miR-374b-3p-1.064THAP9-AS1lncRNAENSG00000251022.61.9542.61E-55
HepG2 24 hourshsa-miR-374b-3p-1.064MALAT1lncRNAENSG00000251562.81.0242.38E-10
HepG2 24 hourshsa-miR-374b-3p-1.064SMAD7mRNAENSG00000101665.91.9991.67E-42
HepG2 24 hourshsa-miR-374b-3p-1.064TIRARPmRNAENSG00000163659.131.0701.55E-18
HepG2 24 hourshsa-miR-769-5p-1.058MALAT1lncRNAENSG00000251562.81.0242.38E-10
HepG2 24 hourshsa-miR-769-5p-1.058SNHG17lncRNAENSG00000196756.131.0931.46E-10
HepG2 24 hourshsa-miR-769-5p-1.058TFAP4mRNAENSG00000090447.121.0101.16E-11
HepG2 24 hourshsa-miR-769-5p-1.058FAXDC2mRNAENSG00000170271.111.5651.25E-13
HepG2 24 hourshsa-miR-4521-4.496MBNL1mRNAENSG00000152601.171.2422.60E-16
HepG2 24 hourshsa-miR-7974-3.432CREB3 L3mRNAENSG00000060566.141.0624.00E-06
HepG2 24 hourshsa-miR-194-3p-1.463ARHGAP24mRNAENSG00000138639.181.2811.19E-08
HepG2 24 hourshsa-miR-194-3p-1.463HOXA1mRNAENSG00000105991.91.1922.48E-06
HepG2 24 hourshsa-miR-27b-5p-1.236OPTNmRNAENSG00000123240.171.5316.35E-20
HepG2 24 hourshsa-miR-44889.059MELTFmRNAENSG00000163975.12-1.0852.72E-05
SNU 6 hourshsa-miR-15a-5p-1.526CHAC1mRNAENSG00000128965.132.2625.79E-28
SNU 6 hourshsa-miR-12135-1.298FOXJ1mRNAENSG00000129654.81.2402.10E-06
SNU 24 hourshsa-miR-29b-1-5p-2.126HEY1mRNAENSG00000164683.171.6922.30E-07
SNU 24 hourshsa-miR-374c-5p-1.884CEBPBmRNAENSG00000172216.61.0025.31E-17
SNU 24 hourshsa-miR-374c-5p-1.884VEGFAmRNAENSG00000112715.221.4482.13E-45
SNU 24 hourshsa-miR-374c-5p-1.884ADMmRNAENSG00000148926.102.3401.78E-143
SNU 24 hourshsa-miR-374c-5p-1.884GAS5lncRNAENSG00000234741.81.7952.73E-202
SNU 24 hourshsa-miR-374c-5p-1.884SNHG5lncRNAENSG00000203875.121.3701.13E-50
SNU 24 hourshsa-miR-374c-5p-1.884SNHG20lncRNAENSG00000234912.121.1761.34E-23
SNU 24 hourshsa-miR-32-3p-1.821YOD1mRNAENSG00000180667.101.6581.06E-111
SNU 24 hourshsa-miR-32-3p-1.821TNFAIP3mRNAENSG00000118503.152.3163.47E-10
SNU 24 hourshsa-let-7b-3p-1.541KLF4mRNAENSG00000136826.151.5721.17E-31
SNU 24 hourshsa-let-7b-3p-1.541ADRB2mRNAENSG00000169252.51.7439.09E-48
SNU 24 hourshsa-let-7b-5p-1.541SNHG12lncRNAENSG00000197989.141.0921.50E-21
SNU 24 hourshsa-let-7b-5p-1.541LMCD1-AS1lncRNAENSG00000227110.71.1895.83E-03
SNU 24 hourshsa-let-7b-5p-1.541CARMNlncRNAENSG00000249669.101.6371.11E-04
SNU 24 hourshsa-miR-195-5p-1.456VEGFAmRNAENSG00000112715.221.4482.13E-45
SNU 24 hourshsa-miR-195-5p-1.456ADRB2mRNAENSG00000169252.51.7439.09E-48
SNU 24 hourshsa-miR-195-5p-1.456SNHG12lncRNAENSG00000197989.141.0921.50E-21
SNU 24 hourshsa-miR-195-5p-1.456EPB41 L4A-AS1lncRNAENSG00000224032.72.1704.36E-34
SNU 24 hourshsa-miR-195-5p-1.456AZIN1-AS1lncRNAENSG00000253320.71.0702.01E-03
SNU 24 hourshsa-miR-195-5p-1.456PVT1lncRNAENSG00000249859.111.2644.13E-79
SNU 24 hourshsa-miR-195-5p-1.456LINC01578lncRNAENSG00000272888.71.4464.59E-72
SNU 24 hourshsa-miR-34a-5p-1.316KLF4mRNAENSG00000136826.151.5721.17E-31
SNU 24 hourshsa-miR-34a-5p-1.316HSPA1BmRNAENSG00000204388.71.6762.82E-82
SNU 24 hourshsa-miR-34a-5p-1.316SNHG7lncRNAENSG00000233016.71.1161.01E-40
SNU 24 hourshsa-miR-57011.535SYNGR3mRNAENSG00000127561.15-1.5051.63E-08
SNU 24 hourshsa-miR-57011.535RFLNBmRNAENSG00000183688.4-1.3401.86E-27
SNU 24 hourshsa-miR-121363.334ACVR2BmRNAENSG00000114739.14-1.0753.21E-13
SNU 24 hourshsa-miR-121363.334NR4A2mRNAENSG00000153234.14-1.7549.17E-06
SNU 24 hourshsa-miR-121363.334CSPG5mRNAENSG00000114646.10-1.0891.71E-08
SNU 24 hourshsa-miR-121363.334AREGmRNAENSG00000109321.11-1.0162.62E-03
SNU 24 hourshsa-miR-121363.334NRARPmRNAENSG00000198435.4-1.7633.74E-12
SNU 24 hourshsa-miR-121363.334MAFmRNAENSG00000178573.7-1.7485.31E-04
SNU 24 hourshsa-miR-44928.427IGFBP5mRNAENSG00000115461.5-1.0732.38E-18
SNU 24 hourshsa-miR-448813.091PDGFBmRNAENSG00000100311.17-1.1011.09E-51
Table 3 Summary of lncRNA-miRNA-mRNA interactions in HepG2 and SNU449 cells at 24 hours of Sorafenib treatment
lncRNA
miRNA
mRNA
Condition
MIR4435-2HGhsa-miR-3187-3pATOH8HepG2 24 hours
MALAT1hsa-miR-769-5pFAXDC2
ZFAS1hsa-miR-193b-3pSIAH1
THAP9-AS1hsa-miR-374b-3pSMAD7
MALAT1hsa-miR-374b-3p
MALAT1hsa-miR-769-5pTFAP4
SNHG17hsa-miR-769-5p
MALAT1hsa-miR-374b-3pTIPARP
SNHG12hsa-let-7b-5pADRB2SNU449 24 hours
EPB41 L4A-AS1hsa-miR-195-5p
PVT1hsa-miR-195-5p
LINC01578hsa-miR-195-5p
GAS5hsa-miR-374c-5pCEBPB
SNHG5hsa-miR-374c-5p
SNHG7hsa-miR-34a-5pKLF4
EPB41 L4A-AS1hsa-miR-195-5pVEGFA
PVT1hsa-miR-195-5p
LINC01578hsa-miR-195-5p
GAS5hsa-miR-374c-5p
SNHG5hsa-miR-374c-5p