miR-10a-5p and miR-10b-5p restore colonic motility in aged mice

Table 1 Oligonucleotides used in this study: Primers

Name	Sequences (5' to 3')	Sense	Product (bp)	Spices	Application
miRTQ	CGAATTCTAGAGCTCGAGGCAGGCGACATGGCTGGCTAGTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCTTTTTTTTTTTTTTTTTTTTTTTTTVN1	Reverse			cDNA
miRTQ-rev	CGAATTCTAGAGCTCGAGGCAGG	Reverse			qPCR
miR-10a	TACCCTGTAGATCCGAATTTG	Forward	123	Mouse/human	qPCR
miR-10b	TACCCTGTAGAACCGAATTTG	Forward	123	Mouse/human	qPCR
Snord55	GGTAATGCTGCATACTCCCGAG	Forward		Human	qPCR
Snord66	CTGAGACCACATGATGGGATTG	Forward		Mouse	qPCR
M13	CAGGAAACAGCTATGAC	Forward	289		Cloning
M13-rev	ACTGGCCGTCGTTTTAC	Reverse	289		Cloning

¹In RTQ primer, V is A, G, or C; N is A, G, C, or T.

Table 2 Oligonucleotides used in this study: MicroRNA mimics

Name	Sequence (5' to 3')	Sense	Spices	Application
miR-10a mimic	UACCCUGUAGAUCCGAAUUUGUG	Sense	Mouse/human	In vivo
	TTAUGGGACAUCUAGGCUUAAAC	Antisense
miR-10b mimic	UACCCUGUAGAACCGAAUUUGUG	Sense	Mouse/human	In vivo
	TTAUGGGACAUCUUGGCUUAAAC	Antisense

Table 3 Body weight and blood glucose levels across different age groups in this study

Group	Age (months)	Blood glucose (mg/dL)	Body weight (g)
Young	2-6	104.5 ± 5.56	26 ± 5.48
Middle	8-14	110.7 ± 13.97	35.5 ± 3.42a
Old	18-30	95.5 ± 12.40	38.9 ± 5.78b

^aP < 0.05, vs young, aged group.

^bP < 0.01, vs young, aged group.

n = 10-12 per group.