Exosomal miR-182 regulates the effect of RECK on gallbladder cancer

Table 1 Primer sequences

	Upstream primer (5'→3')	Downstream primer (5'→3')
miR-182	TGCGGTTTGGCAATGGTAGAAC	CCAGTGCAGGGTCCGAGGT
RECK	TCTGCAGGGGAAGTTGGTTG	CAGTTACAGGGCAGACCTGT
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT
GAPDH	AGAAGGCTGGGGCTCATTTG	AGGGGCCATCCACAGTCTTC

miR-182: MicroRNA 182; RECK: Reversion-inducing-cysteine-rich protein with kazal motifs.

Table 2 Correlation between miR-182 and clinical features

Categories	n	miR-182		χ2	P value
		Low expression	High expression
Gender				0.125	0.819
Male	36	17	25
Female	42	16	20
Age in yr				0.273	0.646
≤ 60	45	19	26
> 60	33	12	21
Smoking history				0.075	0.823
Yes	41	22	19
No	37	21	16
Drinking history				0.396	0.645
Yes	46	22	24
No	32	15	17
N staging				5.128	0.035
N0	25	12	13
N1/N2/N3	53	12	41
M staging				5.349	0.027
M0	22	10	12
M1	56	11	45
RECK expression
Low	31	15	16	5.246	0.028
High	47	11	36

M: Metastasis; N: Node; RECK: Reversion-inducing-cysteine-rich protein with kazal motifs.