Mesencephalic astrocyte-derived neurotrophic factor ameliorates steatosis in HepG2 cells by regulating hepatic lipid metabolism

Table 1 Primers used for quantitative real-time PCR experiments

Gene	Species	Primers
MANF	Homo sapiens	F: 5’-CGGTTGTGCTACTATATCGGGG-3’
		R: 5’-GGCCAGAGGCTTTGATACCT-3’
ACCα	Homo sapiens	F: 5’-ATGTCTGGCTTGCACCTAGTA-3’
		R: 5’-CCCCAAAGCGAGTAACAAATTCT-3’
FAS	Homo sapiens	F: 5’-AGATTGTGTGATGAAGGACATGG-3’
		R: 5’-TGTTGCTGGTGAGTGTGCATT-3’
SREBP-1C	Homo sapiens	F: 5’-CGGAACCATCTTGGCAACAGT-3’
		R: 5’-CGCTTCTCAATGGCGTTGT-3’
HMGCR	Homo sapiens	F: 5’-TGATTGACCTTTCCAGAGCAAG-3’
		R: 5’-CTAAAATTGCCATTCCACGAGC-3’
CD36	Homo sapiens	F: 5’-GGCTGTGACCGGAACTGTG-3’
		R: 5’-AGGTCTCCAACTGGCATTAGAA-3’
FABP-1	Homo sapiens	F: 5’-GTGTCGGAAATCGTGCAGAAT-3’
		R: 5’-GACTTTCTCCCCTGTCATTGTC-3’
β-actin	Homo sapiens	F: 5’-GCCGACAGGATGCAGAAGG-3’
		R: 5’-TGGAAGGTGGACAGCGAGG-3’
MANF	Mus musculus	F: 5’-TCTGGGACGATTTTACCAGGA-3’
		R: 5’-TCTTGCTTCACGGCAAAACTTTA-3’
β-actin	Mus musculus	F: 5’-TCACTGTCCACCTTCCAGCAGATG-3’
		R: 5’-CTCAGTAACAGTCCGCCTAGAAGC-3’

MANF: Mesencephalic astrocyte-derived neurotrophic factor; ACCα: Acetyl-CoA carboxylase α; FAS: Fatty acid synthase; SREBP-1C: Sterol regulatory element binding transcription factor 1C; HMGCR: 3-hydroxy3-methylglutaryl coenzyme A reductase; FABP-1: Fatty acid binding protein-1.