High expression of APC is an unfavorable prognostic biomarker in T4 gastric cancer patients

Table 1 Relationship between APC^high and clinical characteristic and known prognostic genes

Characteristic	APChigh	APClow	P-value
Age	66 ± 8	61 ± 9	0.084
Stage
I	5	25	0.031
II	10	12	1
III + IV	29	7	0.043
Grade
1	4	33	0.031
2	15	5	0.047
3	25	6	0.039
Lymph node metastasis
N0	7	23	0.027
N1	10	12	1
N2	11	4	0.031
N3	16	5	0.035
Distant metastasis
M0	7	9	0.781
M1	37	35	0.812
Gene
KIT	29	15	0.031
PIK3CA	32	12	<0.001
KRAS	28	16	0.038
MLH1	28	16	0.038

APC: Adenoma polyposis coli.

Table 2 Association between differential mRNAs and APC^high

mRNA	P-value (t-test)	P-value (Wilcoxon test)	Fold change	Regulation
BTAF1	1.45E-08	4.81E-10	2.00561	Up-regulation
ARID5B	1.20E-06	1.30E-07	2.011267	Up-regulation
ZNF81	2.04E-06	3.84E-09	2.175277	Up-regulation
JMY	1.60E-09	4.99E-11	2.094035	Up-regulation
MAP3K14	7.83E-07	3.00E-07	2.092268	Up-regulation
PDE4D	5.58E-07	4.33E-08	2.151154	Up-regulation
CRYBG3	4.92E-07	1.10E-07	2.242404	Up-regulation
PARD3B	2.59E-06	9.38E-08	2.007173	Up-regulation
PPARGC1B	1.76E-08	2.04E-09	2.576507	Up-regulation
TRANK1	6.01E-07	1.15E-08	2.165217	Up-regulation
REV3L	6.32E-07	1.14E-09	2.13368	Up-regulation
IL6ST	3.07E-07	1.30E-07	2.168223	Up-regulation

APC: Adenoma polyposis coli.

Table 3 APC^high associated KEGG pathways

MSigDB	Size	ES	NES	P-value	Group
Kegg_b_cell_receptor_signaling_pathway	74	0.579	1.712	0.012	High
Kegg_pancreatic_cancer	70	0.472	1.597	0.025	High
KEGG_PROSTATE_CANCER	89	0.450	1.570	0.025	High
Kegg_prostate_cancer	211	0.396	1.541	0.032	High
Kegg_neurotrophin_signaling_pathway	126	0.437	1.533	0.046	High
Kegg_progesterone_mediated_oocyte_maturation	85	0.412	1.491	0.040	High
Kegg_ascorbate_and_aldarate_metabolism	25	-0.674	-1.851	0.002	Low
Kegg ppar_signaling_pathway	69	-0.456	-1.621	0.003	Low
Kegg_drug_metabolism_other_enzymes	51	-0.590	-1.789	0.003	Low
Kegg_steroid_hormone_biosynthesis	55	-0.595	-1.853	0.004	Low
Kegg_porphyrin_and_chlorophyll_metabolism	41	-0.617	-1.833	0.004	Low
Kegg_drug_metabolism_cytochrome_p450	70	-0.569	-1.776	0.012	Low
Kegg_retinol_metabolism	63	-0.556	-1.687	0.012	Low
Kegg_metabolism_of_xenobiotics_by_cytochrome_p450	68	-0.572	-1.782	0.014	Low
Kegg_pentose_and_glucuronate_interconversions	27	-0.638	-1.734	0.016	Low
Kegg_linoleic_acid_metabolism	29	-0.589	-1.691	0.019	Low
Kegg_alpha_linolenic_acid_metabolism	19	-0.565	-1.662	0.020	Low
Kegg_arachidonic_acid_metabolism	58	-0.424	-1.541	0.031	Low

APC: Adenoma polyposis coli.