Prognostic significance of lymphovascular invasion in colorectal cancer and its association with genomic alterations

Table 1 Comparison of characteristics between colorectal cancer patients with and without lymphovascular invasion

Characteristic	LVI, n = 150	Non-LVI, n = 1069	P value
	No. of patients (%)	No. of patients (%)
Gender
Male	93 (62.0)	621 (58.1)
Female	57 (38.0)	448 (41.9)	0.377
Age in yr
≤ 65	87 (58.0)	629 (58.8)
> 65	63 (42.0)	440 (41.2)	0.860
Tumor site
Colon	72 (48.0)	497 (46.5)
Rectum	78 (52.0)	572 (53.5)	0.793
Histological differentiation
Well/moderately	79 (52.7)	815 (76.2)
Poorly/mucinous	71 (47.3)	254 (23.8)	< 0.001
TNM stage
I	12 (8.0)	207 (19.4)
II	30 (20.0)	368 (34.4)
III	64 (42.7)	358 (33.5)
IV	44 (29.3)	136 (12.7)	< 0.001

LVI: Lymphovascular invasion; TNM: Tumor-node-metastasis.

Table 2 Multivariate Cox analysis for 5-year overall survival in 1219 colorectal cancer patients

Characteristic	HR (95%CI)	P value
Gender
Male	1 (Reference)
Female	0.93 (0.77-1.13)	0.481
Age in yr
≤ 65	1 (Reference)
> 65	1.93 (1.60-2.33)	< 0.001
Tumor site
Colon	1 (Reference)
Rectum	0.94 (0.78-1.13)	0.497
Histological differentiation
Well/moderately	1 (Reference)
Poorly/mucinous	1.51 (1.24-1.85)	< 0.001
TNM stage
I	1 (Reference)
II	1.63 (1.07-2.47)	0.022
III	3.31 (2.24-4.90)	< 0.001
IV	11.88 (7.94-17.77)	< 0.001
LVI
Negative	1 (Reference)
Positive	1.77 (1.40-2.25)	< 0.001

HR: Hazard ratio; CI: Confidence interval; TNM: Tumor-node-metastasis; LVI: Lymphovascular invasion.

Table 3 Top 20 hub genes ranked by closeness, degree, and eigenvector

Rank	Closeness		Degree		Eigenvector
	Gene	Value	Gene	Value	Gene	Value
1	EP300	0.256684	EP300	15	EP300	0.481394
2	NOTCH1	0.241814	PES1	11	ESR1	0.320964
3	ESR1	0.240000	ESR1	10	STAT5A	0.297293
4	AKT1	0.227488	MAPK1	8	STAT3	0.285113
5	DVL1	0.225352	AKT1	8	MAPK1	0.269273
6	STAT5A	0.223776	BRCA1	7	STAT5B	0.240224
7	MED1	0.219680	NHP2L1	7	AKT1	0.221299
8	STAT3	0.213808	STAT5A	6	MED1	0.202291
9	STAT5B	0.213333	MED1	6	PPARA	0.169136
10	SIRT1	0.212860	STAT3	6	BCR	0.166509
11	PPARA	0.210526	CIRH1A	6	HDAC2	0.164826
12	HDAC2	0.209607	SNRPD3	6	IL2RB	0.151029
13	ARNT	0.209607	RPL3	6	KAT2A	0.139521
14	CSNK1E	0.209150	STAT5B	5	HDAC10	0.139521
15	HDAC10	0.206452	BCR	5	SIRT1	0.137321
16	KAT2A	0.206452	SIRT1	5	ARNT	0.124740
17	MYB	0.204691	HDAC2	5	RPS6KB1	0.105985
18	CITED2	0.204691	CDH1	5	NOTCH1	0.094497
19	IGF1R	0.204255	SNRPA1	5	IGF1R	0.087082
20	MAPK1	0.202105	RPS6KB1	4	MYB	0.085425