Low expression of CDK5RAP3 and DDRGK1 indicates a poor prognosis in patients with gastric cancer

Table 1 Relationship between CDK5RAP3 and DDRGK1 protein expression in gastric cancer tissues and various clinicopathological variables

Variables	Total	CDK5RAP3 expression				DDRGK1 expression
		Low	High	χ2	P value	Low	High	χ2	P value
Gender				1.659	0.198			3.057	0.080
Male	107	84	23			74	33
Female	28	25	3			24	4
Age (yr)				4.441	0.035			0.719	0.397
> 60	91	78	13			64	27
≤ 60	44	31	13			34	10
Tumor size(cm)				0.125	0.723			0.043	0.835
> 5	53	42	11			39	14
≤ 5	72	67	15			59	23
Location of tumor				3.860	0.277			1.537	0.674
Lower 1/3	55	48	7			37	18
Middle 1/3	20	15	5			16	4
Upper 1/3	45	36	9			34	11
More than 1/3	15	10	5			11	4
Borrmann type				0.285	0.593			1.312	0.252
I + II Type	31	24	7			25	6
III + IV Type	104	85	19			73	31
Degree of differentiation				0.187	0.666			4.414	0.036
Well/moderate	57	47	10			36	21
Poor and not	78	62	16			62	16
Histological type				1.271	0.736			8.547	0.036
Papillary	58	48	10			39	19
Tubular	42	32	10			27	15
Mucinous	10	9	1			9	1
Signet-ring cell	25	20	5			23	2
Depth of invasion				5.674	0.017			1.428	0.232
T1 + T2	21	13	8			13	8
T3 + T4	114	96	18			85	29
Lymph node metastasis				0.008	0.927			6.023	0.014
Negative	20	16	4			10	10
Positive	115	93	22			88	27
TNM stage				1.383	0.240			2.632	0.105
I + II	44	33	11			28	16
III + IV	91	76	15			70	21
Distant metastasis				1.761	0.184			0.639	0.424
Negative	128	102	26			92	36
Positive	7	7	0			6	1

Table 2 Relationship between different CDK5RAP3 and DDRGK1 protein expression status in gastric cancer tissues and various clinicopathological variables

Variables	Total	C53 and DDRGK1 low expression	C53 and/or DDRGK1 high expression	χ2	P value
Gender
Male	107	59	48	5.077	0.024
Female	28	22	6
Age (yr)
> 60	91	55	36	0.022	0.881
≤ 60	44	26	18
Tumor size (cm)
> 5	53	32	21	0.005	0.943
≤ 5	82	49	33
Location of tumor
Lower 1/3	55	33	22	1.481	0.687
Middle 1/3	20	12	8
Upper 1/3	45	29	16
More than 1/3	15	7	8
Borrmann type
I + II Type	31	20	11	0.342	0.559
III + IV Type	104	61	43
Degree of differentiation
Well/moderate	57	31	26	1.296	0.255
Poor and not	78	50	28
Histological type
Papillary	57	33	25	4.415	0.220
Tubular	42	22	20
Mucinous	10	8	2
Signet-ring cell	25	18	7
Depth of invasion
T1 + T2	21	8	13	4.972	0.026
T3 + T4	114	73	41
Lymph node metastasis
Negative	20	8	12	3.913	0.048
Positive	115	73	42
TNM stage
I + II	44	20	24	5.754	0.016
III + IV	91	61	30
Distant metastasis
Negative	126	75	53	2.034	0.154
Positive	7	6	1

Table 3 Univariate analysis of the correlation between clinicopathological parameters and survival of patients with gastric cancer

Clinicopathological parameters	Three-year cumulative survival rate	Log-rank test	P value
Gender
Male	75.4	0.395	0.53
Female	71.4
Age (yr)
> 60	75.4	0.163	0.686
≤ 60	73.8
Tumor size (cm)
> 5	68.3	0.988	0.32
≤ 5	79.3
Location of tumor
Lower 1/3	78.2	3.438	0.329
Middle 1/3	83.6
Upper 1/3	63.3
More than 1/3	86.7
Borrmann type
I + II	76.9	0.373	0.541
III + IV	74
Degree of differentiation
Well/moderate	75.5	0.111	0.739
Poor and not	74.2
Histological type
Papillary	70	4.386	0.223
Tubular	85.6
Mucinous	70
Signet-ring cell	69.1
Depth of invasion
T1 + T2	91.7	4.95	0.026
T3 + T4	71.7
Lymph node metastasis
Negative	95	4.629	0.031
Positive	71.2
TNM stage
I + II	92.3	11.424	0.001
III + IV	66.3
Distant metastasis
Negative	76.7	8.015	0.005
Positive	42.9
CDK5RAP3 expression
Low	71.1	5.168	0.023
High	90.8
DDRGK1 expression
Low	67.7	5.971	0.015
High	91.9
CDK5RAP3/DDRGK1 expression
CDK5RAP3 and/or DDRGK1 high	90.1	10.415	0.001
CDK5RAP3 and DDRGK1 low	64.2

Table 4 Multivariate analysis of the correlation between clinicopathological parameters and survival time of patients with gastric cancer

Covariates	Coefficient	Standard error	HR	95%CI for HR	P value
CDK5RAP3 expression (high vs low)	-1.226	0.735	0.294	0.070-1.239	0.095
DDRGK1 expression (high vs. low)	-0.979	0.536	0.376	0.131-1.074	0.068
CDK5RAP3 and DDRGK1 expression (low/low vs. high and/or high)	1.178	0.453	3.247	1.336-7.891	0.009
Depth of invasion (T3,T4 vs T1,T2)	1.071	1.045	2.920	0.376-22.635	0.305
Lymph node metastasis (positive vs negative)	1.538	1.020	1.974	0.631-34.367	0.132
Distant metastasis (positive vs negative)	0.861	0.544	2.365	0.815-6.8631	0.113
TNM stage (stage III and IV vs I and II)	-1.630	0.608	7.195	0.060-0.645	0.007