Copyright
©The Author(s) 2017.
World J Gastroenterol. Dec 28, 2017; 23(48): 8512-8525
Published online Dec 28, 2017. doi: 10.3748/wjg.v23.i48.8512
Published online Dec 28, 2017. doi: 10.3748/wjg.v23.i48.8512
Table 1 qPCR primers
| Gene name | Forward primer (F) |
| Reverse primer (R) | |
| U6 | F: 5’-TTCCTCCGCAAGGATGACACGC-3’ |
| R: Universal miRNA qPCR primer | |
| miR-214 | F: 5’-CAGGCACAGACAGGCAGT-3’ |
| R: Universal miRNA qPCR primer | |
| miR-301a | F:5’-GGCAGTGCAATAGTATTGT-3’ |
| R: Universal miRNA qPCR primer | |
| miR-34b | F: 5’-TAGGCAGTGTCATTAGCTGATTG-3’ |
| R: Universal miRNA qPCR primer | |
| miR-31 | F: GCCGCAGGCAAGATGCTGGC |
| R: Universal miRNA qPCR primer | |
| miR-155 | F: TTAATGCTAATCGTGATAGGGGT |
| R: Universal miRNA qPCR primer | |
| miR-200b | F: GCGGCTAATACTGCCTGGT |
| R: Universal miRNA qPCR primer | |
| miR-16 | F: GTAGCAGCACGTAAATATTGGCG |
| R: Universal miRNA qPCR primer | |
| miR-148a | F: AGCAGTTCAGTGCACTACAG |
| R: Universal miRNA qPCR primer | |
| GAPDH | F: 5’-TGTCCCCACTGCCAACGTGTCA-3’ |
| R: 5’-GCGTCAAAGGTGGAGGAGTGGGT-3’ | |
| SOX2 | F: 5’-TACAGCATGTCCTACTCGCAG-3’ |
| R: 5’-GAGGAAGAGGTAACCACAGGG-3’ | |
| NANOG | F: 5’-CCAACATCCTGAACCTCAGCTAC-3’ |
| R: 5’-GCCTTCTGCGTCACACATT-3’ |
- Citation: Wang ZF, Ma DG, Zhu Z, Mu YP, Yang YY, Feng L, Yang H, Liang JQ, Liu YY, Liu L, Lu HW. Astragaloside IV inhibits pathological functions of gastric cancer-associated fibroblasts. World J Gastroenterol 2017; 23(48): 8512-8525
- URL: https://www.wjgnet.com/1007-9327/full/v23/i48/8512.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i48.8512