Basic Study
Copyright ©The Author(s) 2017.
World J Gastroenterol. Oct 21, 2017; 23(39): 7087-7097
Published online Oct 21, 2017. doi: 10.3748/wjg.v23.i39.7087
Table 1 Patient characteristics
Colorectal cancer patients, n = 70
Right colon, n = 28Left colon, n = 34Rectum, n = 8
SexFemale13133
Male15215
Age (mean)76.8972.2670.63
pTT1342
T2692
T316183
T4331
pNN020228
N+812-
StageI9104
IIa9103
IIb111
IIIa13-
IIIb45-
IIIc22-
IV23-
KRAS statusMutant11156
Wild-type17192
Table 2 KRAS mutational status by pyrosequencing
KRAS wild-typeKRAS mutations
Codon 12
Codon 13
Others
G12DG12VG12RG12SG13DG13RA146VA146TA59TQ61RQ61HQ61L
Right colon17201031110110
Left colon19650010111000
Rectum2200110000011
1051151221121
Total38 (54.29%)32 (45.71%)
Table 3 KRAS G12D mutation levels in DNA from stool samples of wild-type control patients
G12D
Wild-type
SingleMergedSingleMerged
PatientTumor locationTumor stagePositive eventsCopies/μLCopies/20 μL reactionPositive eventsCopies/μLCopies/20 μL reactionCopies/ng DNAPositive eventsCopies/μLCopies/20 μL reactionPositiveeventsCopies/μLCopies/20 μL reactionCopies/ng DNA

53LeftI10.1230.071.400.01194918046910.50210.001.615
20.173.41210.8216
0001169.9198
00013612.1242
56LeftIIA10.081.610.020.400.003171.428761.5631.200.240
000252.142
000161.326
000181.428
63RightIIA10.081.610.020.420.00325820.440895720.30406.003.123
00022222.1442
00021719380
00026019.9398
71RightIIIB10.081.630.061.200.009896.71343777.20144.001.108
20.1531047.9158
000936.9138
000917.3146
96LeftIV10.091.830.061.200.009323601332.8456.800.437
10.081.6332.652
10.091.834360
000342.958
Table 4 KRAS G12D mutation levels in DNA from stool samples of patients with KRAS G12D mutated tumors
G12D
Wild-type
Single
Mergedsingle
Merged
PatientTumor locationTumor stagePositive eventsCopies/μLCopies/20 μL reactionPositive eventsCopies/μLCopies/20 μL reactionCopies/ng DNAPositive eventsCopies/μLCopies/20 μL reactionPositive eventsCopies/μLCopies/20 μL reactionCopies/ng DNA
12RectumI40.326.4140.377.400.05115512.625249313.10262.001.819
50.397.815812.5250
50.397.818014.1282
17LeftI1147951900484997.201944.0041.3624684446892019664456.009120.00194.043
124398196049964589160
126999198050944609200
119097194048904619220
29LeftIIIB50.418.2110.306.000.024947.71542577.10142.000.577
30.244.8907.4148
30.255736.2124
30RightI60.5711.4180.5410.800.01723822.845675723.10462.000.728
70.6412.826824.8496
50.438.625121.8436
43RightIIIC60.5911.8190.5911.800.03541842840139344.10882.002.602
80.71449245.5910
50.469.248344.8896
461LeftIIC10.091.830.091.800.0148171402657.80156.001.238
10.091.8978.3166
10.091.8878.2164
51RectumI13120470.9018.000.295129210320605110102.602052.0033.639
120.91812911022040
90.6813.612971022040
1312012301032060
641LeftI00000.000.000.0001411224065312.20244.001.877
00016312.1242
00015411.4228
00019513.3266
70LeftI111.122451.3727.400.036251528256408516294.005880.007.656
241.93833133056100
1012026882915820
75LeftIIA000110.336.600.03230427.254496429.00580.002.788
20.183.632629.8596
90.81633430.1602