Randomized Controlled Trial
Copyright ©The Author(s) 2017.
World J Gastroenterol. Sep 14, 2017; 23(34): 6330-6338
Published online Sep 14, 2017. doi: 10.3748/wjg.v23.i34.6330
Table 1 The three differently expressed circRNAs in human gastric cancer and colorectal cancer samples
CircRNAChromTxStartTxEndcircBaseIDBest_transcriptGene nameCatalog
Chr16: 30740287-30740893|+163074028630740893266NM_006662SRCAPExonic
Hsa_circ_0000745172010764520109225790NM_152904SPECC1Exonic
Hsa_circ_00856168131370262131374017127NM_001247996ASAP1Exonic
Table 2 Primers used for quantitative reverse transcription-polymerase chain reaction analysis of circular RNA and mRNA levels
Target IDPrimer sequence, 5’-3’Product size in bp
Circ_0000745F: GTTGAAAGTAGCCCGAGCAG204
R: ACGTGGCACAGACCTCTCTC
Circ_0085616F: GACTACAACTCGCCCACCAC200
R: TCCATTTCTGGGCCATAATC
Chr16:30740286-30740893|+F: CCTTTGCACCGTATTGTGTG199
R: GGCAGGGTACAGAAATCCAG
GAPDHF: CTCGCTTCGGCAGCACA122
R: AACGCTTCACGAATTTGCGT
Table 3 Relationship of hsa_circ_0000190 expression levels (2-ΔΔCt) in gastric cancer tissues with clinicopathological factors of patients with gastric cancer
Clinicopathological factorTissue hsa_circ_0000745
Plasma hsa_circ_0000745
nMean ± SDP valuemean ± SDP value
Age (yr)0.7570.195
< 60330.77 ± 0.830.90 ± 0.96
≥ 60270.58 ± 0.600.69 ± 0.53
Sex0.2030.252
Male430.59 ± 0.670.68 ± 0.60
Female170.92 ± 0.851.13 ± 1.12
Diameter in cm0.1680.127
≥ 5260.76 ± 0.770.87 ± 0.76
< 5340.63 ± 0.710.76 ± 0.84
Differentiation0.012a0.087
Well20.96 ± 1.100.89 ± 0.44
Moderate250.65 ± 0.710.70 ± 0.66
Poor330.70 ± 0.770.88 ± 0.92
Lymphatic metastasis0.0640.087
Present440.71 ± 0.710.85 ± 0.78
Absent160.61 ± 0.810.69 ± 0.88
TNM stage0.0560.046a
I-II140.63 ± 0.810.60 ± 0.53
III-IV460.70 ± 0.720.87 ± 0.87
CEA in ng/mL0.0770.130
≥ 5100.79 ± 0.720.65 ± 0.59
< 5500.66 ± 0.740.85 ± 0.78