Role of mast cell-miR-490-5p in irritable bowel syndrome

Table 1 Primers for mmu-miR-490-5p and U6

mmu-miR-490-5p	Forward	TGGCGGCCATGGATCTCCAG
	RT	GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCCAC
	Reverse	ATCCAGTGCAGGGTCCGAGG
U6	Forward	CGCTTCACGAATTTGCGTGTGTCAT
	Reverse	GCTTCGGCAGCACATATACTAAAAT

Table 2 Primers for real-time polymerase chain reaction

Gene	Forward/reverse primer	PCR product, bp	Gene bank No.
Tryptase	GCCTCTCCCACCTCCTTATC/GGTATTTCCAGCACACAGCA	145	NM_010781.3
PAR-2	AGTTCTCTGCGTCCATCCTC/GGGTGTTTCTTCTTCGTTCG	139	NM_007974.4
U6	CGCTTCACGAATTTGCGTGTGTCAT/ATCCAGTGCAGGGTCCGAGG	106	NM-0012042741