ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior

Table 1 Association of ANGPTL2 expression with pathological features

Group		n	Gray value of ANGPTL2	T value	P value
Tumor differentiation	High-medium differentiation	17	0.500 ± 0.221	-4.770	0
	Low differentiation	43	0.821 ± 0.240
T stage	T1 + T2	22	0.402 ± 0.198	-5.907	0
	T3 + T4	38	0.803 ± 0.280
Lymph node metastasis	Metastatic	35	0.680 ± 0.210	5.591	0
	Non-metastatic	25	0.404 ± 0.153

ANGPTL2: Angiopoietin-like protein 2.

Table 2 Comparison of the gray value of ANGPTL2 protein in blank control, Lv-NC and Lv-ANGPTL2 PAMC82 gastric cancer cells

Group	ANGPTL2/β-actin
Blank control	0.53 ± 0.02
Lv-NC	0.55 ± 0.02
Lv-ANGPTL2	0.90 ± 0.03a

Compared to the blank control and Lv-NC groups,

^aP < 0.05, n = 3. ANGPTL2: Angiopoietin-like protein 2.

Table 3 Comparison of the gray value of ANGPTL2 protein in blank control, Lv-NC-shRNA and Lv-ANGPTL2-shRNA PAMC82 gastric cancer cells

Group	ANGPTL2/β-actin
Blank control	0.99 ± 0.04
Lv-NC-shRNA	0.95 ± 0.02
Lv-ANGPTL2-shRNA	0.49 ± 0.03a

Compared to the blank control and Lv-NC-shRNA groups,

^aP < 0.05, n = 3. ANGPTL2: Angiopoietin-like protein 2.

Table 4 Stable overexpression of ANGPTL2 contributed to the invasive ability of PAMC82 gastric cancer cells

Group	OD
Blank control	0.279 ± 0.020
Lv-NC	0.281 ± 0.019
Lv-ANGPTL2	0.451 ± 0.008b

Compared to the blank control and Lv-NC groups,

^bP < 0.01, n = 3. ANGPTL2: Angiopoietin-like protein 2.

Table 5 Knockdown of ANGPTL2 weakened the invasive ability of PAMC82 gastric cancer cells

Group	OD
Blank control	0.301 ± 0.021
Lv-NC-shRNA	0.299 ± 0.013
Lv-ANGPTL2-shRNA	0.156 ± 0.018b

Compared to the blank control and Lv-NC-shRNA groups,

^bP < 0.01, n = 3. ANGPTL2: Angiopoietin-like protein 2.