Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 21, 2016; 22(47): 10364-10370
Published online Dec 21, 2016. doi: 10.3748/wjg.v22.i47.10364
ANGPTL2 expression in gastric cancer tissues and cells and its biological behavior
Wei-Zhong Sheng, Yu-Sheng Chen, Chuan-Tao Tu, Juan He, Bo Zhang, Wei-Dong Gao
Wei-Zhong Sheng, Yu-Sheng Chen, Bo Zhang, Wei-Dong Gao, Department of General Surgery, Fudan University Affiliated Zhongshan Hospital, Shanghai 200032, China
Chuan-Tao Tu, Juan He, Department of Digestion, Fudan University Affiliated Zhongshan Hospital, Shanghai 200032, China
Author contributions: Sheng WZ, Chen YS contributed equally to this work; Sheng WZ, Chen YS and Gao WD designed the research; Sheng WZ, Chen YS, Tu CT, He J, Zhang B and Gao WD performed the research; Chen YS, Tu CT, He J and Zhang B contributed new reagents/analytic tools; Sheng WZ and Chen YS analyzed the data; Sheng WZ and Gao WD wrote the paper.
Institutional review board statement: This study was reviewed and approved by the Fudan University Affiliated Zhongshan Hospital Institutional review board.
Conflict-of-interest statement: We declare that there are no conflicts of interest to disclose.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Wei-Dong Gao, MD, Associate Professor, Department of General Surgery, Fudan University Affiliated Zhongshan Hospital, No. 180 Fenglin Road, Shanghai 200032, China. gao.weidong@zs-hospital.sh.cn
Telephone: +86-21-64041990 Fax: +86-21-64041990
Received: July 5, 2016
Peer-review started: July 8, 2016
First decision: August 8, 2016
Revised: August 25, 2016
Accepted: September 14, 2016
Article in press: September 14, 2016
Published online: December 21, 2016
Processing time: 167 Days and 5.3 Hours
Abstract
AIM

To explore expression of angiopoietin-like protein 2 (ANGPTL2) and its effect on biological behavior such as proliferation and invasiveness in gastric cancer.

METHODS

Western blotting was used to detect expression of ANGPTL2 in 60 human normal gastric tissues, 60 human gastric cancer tissues and gastric cell lines including GES-1, N87, SGC7901, BGC823 and PAMC82. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assay were used to detect the proliferation and invasive ability of gastric cancer cells.

RESULTS

Compared to normal tissues, ANGPTL2 protein levels were significantly upregulated in gastric tissues, and this level was closely correlated with gastric tumor grade, clinical stage and lymph node metastasis. Compared to GES-1 cells, ANGPTL2 mRNA and protein levels were significantly increased in gastric cancer cells including N87, SGC7901, BGC823 and PAMC82. The expression of ANGPTL2 in highly malignant gastric cancer cell lines BGC823 and PAMC82 was significantly higher than in low malignancy gastric cancer cell lines N87 and SGC7901. MTT and Transwell experiments indicated that the proliferation rate and invasive ability of stable overexpressed gastric cancer cells was faster than in cells transfected with Lv-NC and blank control cells, and the invasive ability of stable overexpressed gastric cancer cells was higher than that of cells transfected with Lv-NC and blank control cells.

CONCLUSION

ANGPTL2 contributed to proliferation and invasion of gastric cancer cells. In clinical treatment, ANGPTL2 may become a new target for treatment of gastric cancer.

Keywords: Gastric cancer; Angiopoietin-like protein 2; Cell invasion; Cell proliferation

Core tip: Expression of angiopoietin-like protein 2 (ANGPTL2) was significantly elevated in gastric cancer tissues and cells. The higher the level of malignancy of the cancer, the higher the expression of ANGPTL2 became. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays estimated that the proliferative and invasive ability of lentivirus-infected gastric cancer cells were evidently improved compared to the Lv-NC and blank control groups, which indicate that ANGPTL2 was conducive to the proliferation and invasion of gastric cancer cells.