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©The Author(s) 2016.
World J Gastroenterol. Nov 21, 2016; 22(43): 9506-9514
Published online Nov 21, 2016. doi: 10.3748/wjg.v22.i43.9506
Published online Nov 21, 2016. doi: 10.3748/wjg.v22.i43.9506
Table 1 Overview of detected chromosomal aberrations by chromosomal comparative genome hybridization and array comparative genome hybridization on gastric cancer cell lines
| Cell line | cCGH | aCGH |
| ACP02 | +8p21-pter, +8q24, +9p12-p22, +9q21.1-q21.3, +15q11.1-q14, +16p, +16q, +17p11, +17q11.2, +17q23, +22q11.1-q12.3 | +1p13.2, +1p21.3, +1q21.2, +1q21.3, +2p11.2, +4p12, +4p13, +4q12, +5q12.1, +7q11.22, +7q11.23, +8p11.23, +8p21.3, +8q24.11, +8q24.22, -8q24.22, +8q24.23, +8q24.3, +9p13.2, +9p13.3, +9p11.3, +9q21.12, +10p11.22, +10p12.1, +10p12.31, +10p13, +10p14, +10q11.21, -11p11.12, +11p11.2, , -11q12.1, -11q12.2, -11q12.3, +12q12-q15, +13q12.11, +14q11.2, +15q11.2, +15q12, +15q14, +15q15.3, +15q24.1, +15q25.1, +15q26.1, +16p11.2, +16p12.3, +16q11.2, +16q13, +16q21, +16q22.1, +17p11.2, +17q11.2, +17q23.1, +18q11.2, +19q13.11, +20p11.1, +20p11.23, +20p12.2, +20q11.21, +20q11.23, +22q11.21,+ 22q11.23, +22q12.2, +22q12.3 |
| ACP03 | +4p15.1-pter, +6p22.3-p24, +6q25.1,q26, +8p22-pter, -8q11.1-q11.2, +9p12-p22, +10p12-p14, -11p11.1, -11q12, +15q11.1-q15, +15q23-q26.1, +16p12-p13.1, +16p21-p23, +22q11.1-q12.1 | +1p13.2, +1p13.3, +1p21.3, +1q21.1, +1q21.2, 1q21.3, +2p12, +4p12, +4p14-p13, +4p15.1, +5q12.1, +6p24.2, +7q11.21, +7q11.22, +7q11.23, +8p11.21, +8p11.23-p11.22, +8p21.2, +8p21.3, -8q11.21, +8q24.11, -8q24.12, +8q24.13, +8q24.21, +8q24.22, -8q24.22, +8q24.23, +8q24.3, +9p13.2, +9p13.3, +9q21.13, +9p23, +10p11.21, +10p11.22, +10p12.1, +10p12.2, +10p12.31, +10p12.33, +10p13, +10p14, +10q11.21, -11p11.12, +11q11.2, -11q12.1-q12.2, -11q12.3, +12q12-q15, +14q11.2, +14q13.2, +15q11.2, +15q12, +15q13.1, +15q14, +15q24.1, +15q25.1, +15q25.2, +15q26.1, +16p11.2, +16p12.3, +16q11.2, +16q12.1, +16q12.2, +16q21, +16q22.1, +17p11.2, +17q11.2, +17q23.1, +18q11.2, +18q12.1, +19q13.11, +20p11.1, +20p11.23, +20p12.2, +20q11.21, +20q11.22, +20q11.23, +22q11.21, +22q11.23, +22q12.2, +22q12.3 |
| AGP01 | +1p13-21, +1q12-q21.3, +2p11.2-p12, +4p11-p12, +4q12-q13.1, +5p11-p12, +5q11.2-q12, +6p11-p12, +6q12-q16.1, +7q11.1-q11.2, +9p12-p13, +9q13-q21.3, +10p11.2-p12.3, +10q11.1-q21.1, +11p11-p11.2, +12p11.1, +12q12, +13q11-q12, +14q11.1-q13, +15q11-q14, +16p11.2, +16q12, +17p11.2, +17q11.2, +18p11.2-p11.3, +18q11-q12, +19q12-q13.1, +20p11.2-p12, +20q11.1-q11.2 | +1p13.3, +1p21.3, +1q21.2, +1q21.3, +2p11.2, +2p12, +4p12, +4p14-p13, +5p12, +5q12.1, +6p24.2, +6q13, +7q11.21, +7q11.22, +7q11.23, +8p11.21, +8p11.23-p11.22, +8p21.3, -8q11.21, +8q24.11, -8q24.12, +8q24.21, +8q24.22, +8q24.23, +8q24.3, +9p13.2, +9p13.3, +9p22.3, +9q21.12, +9q21.13, +10p11.22, +10p12.1, +10p12.31, +10p13, +10p14, +10q11.21, -11p11.12, +11p11.2, -11q12.1, -11q12.2, -11q12.3, +12q12-q15, +13q12.11, +14q11.2, +15q12, +15q14, +15q15.1, +15q15.3, +15q24.1, +15q25.1, +15q25.2, +15q25.3, +15q26.1, +16p11.2, +16p12.3, +16q11.2, +16q12.1, +16q13, +16q21, +16q22.1, +17p11.2, +17q11.2, +17q23.1, +18q11.2, +19q13.11, +20p11.21, +20p11.1, +20p11.23, +20p12.2, 20q11.21, +20q11.23, +22q11.21, +22q11.23, +22q12.2, +22q12.3 |
| PG100 | +9p12-p23 | +1p13.2, +1p13.3, +1p21.3, +1q21.1, +1q21.2, +1q21.3, +2p11.2, +4p15.1, +6q13, +7q11.21, +7q11.22, +7q11.23, +8p11.21, +8p11.23-p11.22, +8p21.2, +8p21.3, -8q11.21, +8q24.11, +8q24.13, +8q24.22, -8q24.22, +8q24.23, +8q24.3, +9p13.2, +9p13.3, +9p22.3, +9q21.12, +9q21.13, +10p11.22, +10p12.1, +10p12.2, +10p12.31, +10p14, +10q11.21, +11p11.2, -11q12.1, -11q12.2, -11q12.3, +12q12-q15, +13q12.11, +14q11.2, +15q11.2, +15q12, +15q14, +15q15.1, +15q15.3, +15q24.1, +15q25.2, +15q25.3, +15q26.1, +16p11.2, +16p12.3, +16q11.2, +16q12.1, +16q21, +16q22.1, +17p11.2, +17q11.2, +17q23.1, +18q11.2, +19q13.11, +20p11.1, +20p11.23, +20p12.2, +20q11.21, +20q11.23, +22q11.21, +22q11.23, +22q12.1-q12.2, +22q12.2, +22q12.3 |
Table 2 Number of copies of 9q13 locus by FISH analysis in gastric cancer cell lines n (%)
| Cell line | 0 signal | 1 signal | 2 signals | 3 signals | 4 signals | ≥5 signals |
| ACP02 | 12 (6.0) | 26 (13.0) | 112 (56.0) | 25 (12.5) | 22 (11.0) | 3 (1.5) |
| ACP03 | 22 (11.0) | 40 (20.0) | 99 (49.5) | 22 (11.0) | 6 (3.0) | 1 (0.5) |
| AGP01 | 19 (9.5) | 45 (22.5) | 99 (49.5) | 27 (13.5) | 10 (5.0) | - |
| PG100 | 18 (9.0) | 55 (27.5) | 87 (43.5) | 32 (16.0) | 7 (3.5) | 1 (0.5) |
| Control | 34 (17.0) | 68 (34.0) | 97 (48.5) | 1 (0.5) | - | - |
Table 3 MELK and IL11RA gene copy number and clinicopathological features of 68 gastric cancer patients
| MELK | IL11RA | |||||
| 2 copies (n = 38) | ≥3 copies (n = 30) | P value | 2 copies (n = 55) | ≥3 copies (n = 13) | P value | |
| Age (yr) (mean ± SD) | ||||||
| > 50 (64.5 ± 6.9) | 23 | 25 | 0.0748 | 39 | 9 | 0.7461 |
| ≤ 50 (42.5 ± 5.2) | 15 | 5 | 16 | 4 | ||
| Gender | ||||||
| Male | 23 | 21 | 0.5781 | 34 | 9 | 0.7544 |
| Female | 15 | 9 | 21 | 4 | ||
| Histopathology | ||||||
| Intestinal | 23 | 20 | 0.7886 | 32 | 11 | 0.1110 |
| Diffuse | 15 | 10 | 23 | 2 | ||
| Depth of tumor invasion | ||||||
| pT1-pT2 | 10 | 11 | 0.5137 | 18 | 3 | 0.4076 |
| pT3-pT4 | 28 | 19 | 36 | 10 | ||
| Lymph node metastasis | ||||||
| Absent | 10 | 7 | 1.0000 | 11 | 6 | 0.1091 |
| Present | 28 | 23 | 44 | 7 | ||
| Stage | ||||||
| I-II | 25 | 17 | 0.6049 | 33 | 9 | 0.7525 |
| III-IV | 13 | 13 | 22 | 4 | ||
- Citation: Calcagno DQ, Takeno SS, Gigek CO, Leal MF, Wisnieski F, Chen ES, Araújo TMT, Lima EM, Melaragno MI, Demachki S, Assumpção PP, Burbano RR, Smith MC. Identification of IL11RA and MELK amplification in gastric cancer by comprehensive genomic profiling of gastric cancer cell lines. World J Gastroenterol 2016; 22(43): 9506-9514
- URL: https://www.wjgnet.com/1007-9327/full/v22/i43/9506.htm
- DOI: https://dx.doi.org/10.3748/wjg.v22.i43.9506