Fecal microbiota in pouchitis and ulcerative colitis

Table 1 Demographic and clinical characteristics of the subjects

	Healthy controls	UC (n = 41)				Pouch (n = 19)
		Remission	Mild	Moderate	Severe	Pouchitis	NP
Number of patients	16	10	11	10	10	8	11
Sex, n, M/F	9/7	5/5	7/4	5/5	6/4	5/3	5/6
Age (yr)	46.2 ± 10.5	41.8 ± 9.2	43.9 ± 10.5	46 ± 8.2	40.8 ± 11.3	44.9 ± 15.6	47.8 ± 13.2
UC duration (yr)	NA	5.0 ± 1.6	7.0 ± 0.9	5.2 ± 1.7	4.0 ± 2.7	NA	NA
Pouch duration (yr)	NA	NA	NA	NA	NA	2.8 ± 1.5	3.9 ± 2.2
BMI (kg/m2)	24.5 ± 2.3	25.2 ± 1.6	24.8 ± 3.5	23.9 ± 2.7	24.4 ± 3.1	24.9 ± 2.2	24.3 ± 3.5
Mayo score	NA	≤ 2	4.2 ± 0.7	8.5 ± 1.2	11.5 ± 0.3	≥ 7	< 7
Age at colectomy	NA	NA	NA	NA	NA	40.6 ± 12.9	42.4 ± 9.22
Standard medication (%)	NA	0 (0)	5 (46.0)	8 (80.0)	10 (100)	8 (100)	3 (27.0)
Smoking (% at recruitment)	10 (62.5)	4 (40.0)	3 (27.3)	2 (20.0)	3 (30.0)	4 (50.0)	5 (45.5)
Previous number of episodes of pouchitis (%)	NA	NA	NA	NA	NA	4 (50.0)	3 (27.0)
Number of patients with chronic pouchitis (%)	NA	NA	NA	NA	NA	3 (37.5)	2 (18.0)
Secondary causes of pouchitis (%)	NA	NA	NA	NA	NA	2 (25.0)	2 (19.0)

UC: Ulcerative colitis; NA: Not available; Pouch: Ileal pouch established during ileal pouch-anal anastomosis surgery; pouchitis: Inflammation of ileal pouch.

Table 2 Dice analysis of healthy control and ulcerative colitis subgroups

Group	Control	Remission	Mild	Moderate	Severe
Control	(49.79 ± 11.24)%a	(35.32 ± 14.86)%	(30.13 ± 11.23)%	(31.98 ± 16.48)%	(28.18 ± 14.99)%
Remission		(42.89 ± 18.29)%a	(32.79 ± 13.68)%	(30.22 ± 15.28)%	(26.28 ± 13.94)%
Mild			(41.83 ± 16.38)%a	(29.89 ± 13.10)%	(28.31 ± 18.39)%
Moderate				(43.45 ± 21.32)%a	(28.88 ± 13.69)%
Severe					(37.12 ± 19.98)%a

^aP < 0.05; result from similarity in the same group vs similarity in different groups.

Table 3 Dice analysis of healthy control and pouchitis subgroups

Group	Control	Pouchitis	NP
Control	(49.79 ± 11.24)%a	(25.33 ± 11.13)%	(28.86 ± 14.23)%
Pouchitis		(35.43 ± 13.30)%a	(20.87 ± 12.31)%
NP			(35.39 ± 10.80)%a

^aP < 0.05; result from similarity in the same group vs similarity in different groups. NP: No pouchitis.

Table 4 Bacterial diversity comparison

	Healthy controls	UC				Pouch
		Remission	Mild	Moderate	Severe	NP	Pouchitis
Healthy controls	-	0.298	0.006a	0.001a	0.000a	0.000a	0.000a
Remission UC	0.298	-	0.113	0.020a	0.000a	0.014a	0.003a
Mild UC	0.006a	0.113	-	0.404	0.019a	0.007a	0.125
Moderate UC	0.001a	0.020a	0.404	-	0.128	0.009a	0.448
Severe UC	0.000a	0.000a	0.019a	0.128	-	0.019a	0.496
NP: PDAI < 7	0.000a	0.014a	0.007a	0.009a	0.019a	-	0.034a
Pouchitis: PDAI ≥ 7	0.000a	0.003a	0.005a	0.448	0.496	0.034a	-

^aP < 0.05. NP: No pouchitis; UC: Ulcerative colitis; Pouch: Ileal pouch established during ileal pouch-anal anastomosis surgery; pouchitis: Inflammation of ileal pouch; PDAI: Pouchitis Disease Activity Index.