Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Jul 21, 2015; 21(27): 8314-8325
Published online Jul 21, 2015. doi: 10.3748/wjg.v21.i27.8314
Table 1 Histologic grading of intestinal ischemia-reperfusion injury
GradeFeatures
0Normal,villus to crypt ratio 5-6:1, minimal number of lymphocytes and plasma cells, tall columnar surface epithelial cells
1Epithelial cell degenerative changes (cuboidal,vacuolated) but intact, mild increase of lymphocytes and plasma cells in lamina propria
2Decreased villus height, yielding villus to crypt ratio ≤ 1, epithelial cell necrosis or erosions, more chronic inflammation in lamina propria ± neutrophils, glandular dilatation
3Villi effaced (flat surface), epithelial cell necrosis or erosions, pseudomembrane may appear on surface, glandular destruction, inflammation extending deep to muscle layer
4Transmural changes (all of above plus change in muscle layer)
Table 2 Concentrations of inflammatory factors in serum (mean ± SD)
Group(n = 8)p65 (pg/mL)IL-6 (pg/mL)TNF-α(pg/mL)
Sham104.64 ± 11.8950.02 ± 6.3343.79 ± 4.18
Control228.53 ± 24.85a104.91 ± 31.18a70.81 ± 6.97a
Anti-HMGB1145.00 ± 33.63ab62.28 ± 6.73ab52.76 ± 5.71ab
Anti-MyD88191.12 ± 13.22abc85.90 ± 17.37abc63.19 ± 5.47abc
Anti-TRIF183.73 ± 10.81abc78.14 ± 7.38abc59.70 ± 4.63abc
Table 3 Levels of myeloperoxidase activity in the lung and liver (mean ± SD)
Group (n = 4)LungLiver
Sham0.30 ± 0.120.25 ± 0.03
Control0.76 ± 0.06a0.68 ± 0.08a
Anti-HMGB10.46 ± 0.06ab0.40 ± 0.07ab
Anti-MyD880.63 ± 0.03abc0.55 ± 0.03abc
Anti-TRIF0.57 ± 0.06abc0.50 ± 0.04abc
Table 4 Lung injury score in all groups (mean ± SD)
Group (n = 6)Alveolar congestionHemorrhageNeutrophil infiltrationAlveolar wall thicknessTotal score
Sham1.18 ± 0.210.63 ± 0.270.23 ± 0.080.17 ± 0.082.22 ± 0.64
Control3.82 ± 0.182.72 ± 0.181.85 ± 0.152.37 ± 0.2310.75 ± 0.73a
Anti-HMGB12.05 ± 0.121.02 ± 0.260.47 ± 0.100.55 ± 0.144.08 ± 0.60ab
Anti-MyD883.07 ± 0.162.13 ± 0.260.87 ± 0.181.08 ± 0.237.15 ± 0.77abc
Anti-TRIF2.93 ± 0.161.92 ± 0.180.77 ± 0.141.02 ± 0.166.63 ± 0.62abc
Table 5 Intestine injury score in all groups (mean ± SD)
Group (n = 6)JejunumIleum
Sham0.17 ± 0.080.22 ± 0.12
Control2.97 ± 0.16a3.15 ± 0.32a
Anti-HMGB11.23 ± 0.23ab1.32 ± 0.27ab
Anti-MyD882.17 ± 0.22abc2.18 ± 0.29abc
Anti-TRIF2.08 ± 0.17abc2.05 ± 0.27abc
Table 6 HMGB1and NF-κB mRNA expression in lungs from all groups (mean ± SD)
Group (n = 6)HMGB1NF-κB
Sham1.04 ± 0.191.03 ± 0.21
Control2.67 ± 0.30a2.04 ± 0.29a
Anti-HMGB11.89 ± 0.18ab1.42 ± 0.23ab
Anti-MyD882.35 ± 0.31abc1.77 ± 0.18abc
Anti-TRIF2.29 ± 0.28abc1.70 ± 0.13abc
Table 7 HMGB1, NF-κB, MyD88, and TRIF mRNA expression in ileum from all groups (mean ± SD)
Group (n = 6)HMGB1NF-κBMyD88TRIF
Sham1.04 ± 0.191.03 ± 0.211.01 ± 0.101.01 ± 0.11
Control2.67 ± 0.30a2.04 ± 0.29a2.55 ± 0.16a3.05 ± 0.10a
Anti-HMGB11.89 ± 0.18ab1.42 ± 0.23ab2.37 ± 0.08ab2.90 ± 0.13ab
Anti-MyD882.35 ± 0.31abc1.77 ± 0.18abc2.30 ± 0.16ab3.04 ± 0.13ac
Anti-TRIF2.29 ± 0.28abc1.70 ± 0.13abc2.53 ± 0.07acd2.85 ± 0.12abd