MicroRNA-185 regulates expression of lipid metabolism genes and improves insulin sensitivity in mice with non-alcoholic fatty liver disease

Table 1 The primer sequences

SREBP1

5'-ACC CCT GTG TTA GGC TAC CCC AGC CCT C-3'

5'-TCT CCG CAT CTA CGA CCA GTG GGA CTG T-3'

427 bp

SREBP2

5'-TCA AAC TCA GCT GCA ACA ACA GAC GGT A-3'

5'-AAT GAT ATT ATG GGT TGT CCG CCT TTC T-3'

592 bp

GAPDH

5'-TGC CAA ATA TGA TGA CAT CAA GAA GGT G-3'

5'-GTC ATA CCA GGA AAT GAG CTT GAC AAA G-3'

190 bp

FAS

5'-CTG GCT ACC TGA GCA TAG TGT GGA AGA C-3'

5'-TGC AGT GTG TAC AGC TTC TGC CTG TGG G-3'

544 bp

HMGCR

5'-ACA ATA AGA TCT GTG GTT GGA ATT ATG A-3'

5'-CCT AAA ATT GCC ATT CCA CGA GCA ATA T-3'

376 bp

SREBP1: Sterol-regulatory element-binding proteins-1; SREBP2: Sterol-regulatory element-binding proteins-2; GAPDH: Reduced glyceraldehyde-phosphate dehydrogenase; FAS: Fatty acid synthase; HMGCR: 3-hydroxy-3-methylglutaryl-CoA reductase.