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©2014 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 7, 2014; 20(13): 3597-3608
Published online Apr 7, 2014. doi: 10.3748/wjg.v20.i13.3597
Published online Apr 7, 2014. doi: 10.3748/wjg.v20.i13.3597
Table 1 Main clinicopathological features of primary biliary cirrhosis with interface hepatitis and autoimmune hepatitis
PBC1 (n = 41) | AIH (n = 43) | |
Gender (male:female) | 5:36 | 4:39 |
Age (yr, mean ± SD) | 59.0 ± 13.3 | 61.7 ± 10.8 |
Laboratory data | ||
Ratio of AMA(+) or M2(+) and titer of AMA | 75% and 141.2 ± 211.4 | 7% and 0 ± 0 |
Ratio of ANA(+) and titer2 | 72% and 724.3 ± 1262.4 | 91% and 411.1 ± 550.8 |
AST (IU/L) | 51.6 ± 29.7 | 219.5 ± 180.5 |
ALT (IU/L) | 54.0 ± 40.5 | 261.8 ± 253.4 |
ALP (IU/L) | 615.5 ± 572.8 | 449.9 ± 251.8 |
IgG (mg/dL) | 1832.9 ± 607.2 | 2246.2 ± 673.0 |
IgM (mg/dL) | 300.9 ± 220.6 | 193.7 ± 120.0 |
IgA (mg/dL) | 307.9 ± 122.4 | 387.2 ± 183.1 |
Table 2 Primary antibodies used in this study
Primary antibody | Source | Clone | Animal | Type | Pretreatment | Dilution |
CD3 | Nichirei | PS1 | Mouse | Mono | MW3 | 1:1 |
CD4 | Nichirei | 4B12 | Mouse | Mono | MW3 | 1:1 |
CD8 | Dako | C8/144B | Mouse | Mono | MW1 | 1:20 |
CD20 | Dako | L26 | Mouse | Mono | - | 1:400 |
CD38 | Epitomics | EPR4106 | Rabbit | Mono | AC2 | 1:800 |
CD138 | Dako | ML15 | Mouse | Mono | MW3 | 1:100 |
IgG | Dako | A57H | Mouse | Mono | - | 1:200 |
IgM | Nichirei | R1/69 | Mouse | Mono | MW3 | 1:1 |
IgA | Novocastra | N1CLA | Mouse | Mono | PK | 1:300 |
Table 3 Degree of mononuclear cell infiltration with respect to immunological phenotypes at the interface and in the hepatic lobules
PBC1 | AIH | P value | |
At the interface; scores of infiltrating mononuclear cells positive for | |||
CD3 | 2.8 ± 1.2 (3)2 | 4.1 ± 1.1 (4) | 0.0002 |
CD4 | 1.5 ± 1.0 (1) | 2.0 ± 1.2 (2) | 0.0387 |
CD8 | 1.5 ± 0.9 (1) | 2.7 ± 1.7 (3) | 0.0048 |
CD20 | 0.7 ± 0.6 (1) | 1.0 ± 1.0 (1) | 0.0046 |
CD38 | 2.7 ± 1.5 (3) | 3.7 ± 1.3 (4) | 0.0046 |
IgG | 1.3 ± 1.0 (1) | 1.8 ± 1.3 (2) | 0.0301 |
IgM | 1.0 ± 0.8 (1) | 0.6 ± 0.8 (0.5) | 0.0249 |
IgA | 0.5 ± 0.6 (0) | 0.6 ± 0.7 (1) | 0.4486 |
In the hepatic lobules; scores of infiltrating mononuclear cells positive for | |||
CD3 | 2.2 ± 0.9 (2) | 3.3 ± 1.3 (3) | 0.0016 |
CD4 | 0.8 ± 0.9 (0.5) | 1.8 ± 1.1 (2) | 0.0002 |
CD8 | 0.8 ± 0.8 (1) | 2.5 ± 1.5 (2) | < 0.0001 |
CD20 | 0.3 ± 0.6 (0) | 0.7 ± 0.8 (0.5) | 0.0147 |
CD38 | 1.1 ± 1.0 (1) | 2.0 ± 1.2 (2) | 0.0025 |
IgG | 0.6 ± 0.8 (0) | 1.4 ± 1.3 (1) | 0.0007 |
IgM | 0.4 ± 0.5 (0) | 0.6 ± 0.7 (0.5) | 0.166 |
IgA | 0.3 ± 0.7 (0) | 0.6 ± 0.7 (0.5) | 0.0239 |
Table 4 Clinicopathological features of primary biliary cirrhosis based on the predominant immunoglobulin class of the plasma cell
No. of cases(female:male) | Group A28 (25:3) | Group B12 (10:2) | P value |
Age (yr) | 56.1 ± 14.5 (56) | 66.0 ± 7.2 (66) | 0.0479 |
Laboratory data | |||
AST (IU/L) | 50.5 ± 31.2 (43.5) | 55.0 ± 28.3 (42.5) | 0.5839 |
ALT (IU/L) | 54.2 ± 43.6 (45.5) | 55.0 ± 35.4 (46.5) | 0.7238 |
ALP (IU/L) | 681.7 ± 655.0 (446) | 389.1 ± 136.3 (358.5) | 0.4166 |
IgG (mg/dL) | 1737.4 ± 510.4 (1600) | 2081.3 ± 784.4 (2062.5) | 0.2658 |
IgM (mg/dL) | 340.78 ± 219.0 (280.5) | 197.3 ± 198.7 (133.5) | 0.0077 |
IgA (mg/dL) | 309.2 ± 129.8 (281) | 304.6 ± 107.0 (268.5) | 0.8184 |
AMA, M2 (positive ratio) | 80.10% | 55.60% | 0.1916 |
ANA (positive ratio) | 65.40% | 80.00% | 0.6880 |
Histological findings (scores) | |||
Interface hepatitis1 | 2.4 ± 0.6 (2) | 2.8 ± 0.6 (3) | 0.0688 |
Lobular hepatitis2 | 2.3 ± 0.6 (2) | 2.3 ± 0.6 (2) | 0.8577 |
Infiltration of mononuclear cells positive for (scores) | |||
CD3 at the interface3 | 2.7 ± 1.2 (2.5) | 2.8 ± 1.3 (3) | 0.7039 |
CD38 at the interface3 | 2.4 ± 1.5 (2) | 3.3 ± 1.3 (4) | 0.0205 |
CD3 in the hepatic lobules3 | 2.1 ± 0.8 (2) | 2.4 ± 1.2 (2) | 0.5341 |
CD38 in the hepatic lobules3 | 0.9 ± 0.9 (1) | 1.6 ± 1.1 (2) | 0.0497 |
Table 5 Correlation between the elevations in aspartate aminotransferase and the scores of hepatitis and mononuclear cell infiltration of primary biliary cirrhosis and autoimmune hepatitis
Scores of inflammatory cells at the interface vs elevation of aspartate aminotransferase | Primary biliary cirrhosis1 | Autoimmune hepatitis | ||
r | P value | r | P value | |
Scores of interface hepatitis | 0.3600 | 0.0286 | 0.1427 | 0.3796 |
Scores of lobular hepatitis | 0.4056 | 0.0128 | 0.4444 | 0.0041 |
Scores of CD3+ cells at the interface | 0.4172 | 0.0114 | 0.1540 | 0.4724 |
Scores of CD3+ in the lobules | 0.3487 | 0.0371 | 0.4306 | 0.0357 |
Scores of CD4+ at the interface | 0.4410 | 0.0071 | -0.1122 | 0.6017 |
Scores of CD4+ in the lobules | 0.4478 | 0.0062 | 0.3033 | 0.1496 |
Scores of CD8+ at the interface | 0.3466 | 0.0384 | 0.3051 | 0.1471 |
Scores of CD8+ in the lobules | 0.1802 | 0.2929 | 0.7074 | 0.0001 |
Scores of CD38+ at the interface | 0.6010 | 0.0001 | 0.0969 | 0.6601 |
Scores of CD38+ in the lobules | 0.3821 | 0.0215 | 0.1541 | 0.4827 |
Scores of IgG+ at the interface | 0.3909 | 0.0184 | 0.1381 | 0.5200 |
Scores of IgM+ at the interface | 0.5436 | 0.0006 | -0.1372 | 0.5225 |
Table 6 Comparison of the degree of hepatitis and mononuclear cell infiltration in autoantibody positive and negative groups in primary biliary cirrhosis and autoimmune hepatitis
PBC | AMA(+) or M2(+) | AMA(-) and M2(-) | P value |
Comparison between AMA , M2 positive and negative group in PBC | |||
Scores of interface hepatitis1 | 2.41 ± 0.64 (2) | 2.33 ± 0.50 (2) | 0.9120 |
Scores of CD3+ cells at interface2 | 2.62 ± 1.13 (2) | 2.44 ± 1.24 (3) | 0.7552 |
Scores of CD38+ cells at interface2 | 2.42 ± 1.39 (2.5) | 2.33 ± 1.22 (2) | 0.9843 |
Scores of IgG+ cells at interface2 | 1.12 ± 0.59 (1) | 1.22 ± 1.64 (1) | 0.4977 |
Scores of IgM+ cells at interface2 | 1.00 ± 0.63 (1) | 0.89 ± 0.93 (1) | 0.4196 |
Comparison between ANA positive and negative group in PBC | |||
Scores of interface hepatitis1 | 2.42 ± 0.65 (2) | 2.30 ± 0.48 (2) | 0.6241 |
Scores of CD3+ cells at interface2 | 2.47 ± 1.16 (2) | 2.80 ± 1.23 (2) | 0.4548 |
Scores of CD38+ cells at interface2 | 2.43 ± 1.16 (2) | 2.50 ± 1.18 (3) | 0.8113 |
Scores of IgG+ cells at interface2 | 1.09 ± 1.08 (1) | 1.30 ± 0.67 (1) | 0.4193 |
Scores of IgM+ cells at interface2 | 0.83 ± 0.65 (1) | 1.30 ± 0.82 (1) | 0.0742 |
Comparison between ANA positive and negative group in AIH | |||
Scores of interface hepatitis1 | 2.70 ± 0.64 (3) | 2.33 ± 0.58 (2) | 0.3543 |
Scores of CD3+ cells at interface2 | 4.21 ± 1.13 (4) | 3.33 ± 1.53 (3) | 0.2965 |
Scores of CD38+ cells at interface2 | 3.89 ± 1.18 (4) | 2.33 ± 1.15 (3) | 0.0692 |
Scores of IgG+ cells at interface2 | 1.95 ± 1.39 (2) | 1.00 ± 1.00 (1) | 0.2552 |
Scores of IgM+ cells at interface2 | 0.63 ± 0.76 (1) | 0.00 ± 0.00 (0) | 0.1141 |
- Citation: Kobayashi M, Kakuda Y, Harada K, Sato Y, Sasaki M, Ikeda H, Terada M, Mukai M, Kaneko S, Nakanuma Y. Clinicopathological study of primary biliary cirrhosis with interface hepatitis compared to autoimmune hepatitis. World J Gastroenterol 2014; 20(13): 3597-3608
- URL: https://www.wjgnet.com/1007-9327/full/v20/i13/3597.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i13.3597