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©2012 Baishideng Publishing Group Co.
World J Gastroenterol. May 14, 2012; 18(18): 2270-2279
Published online May 14, 2012. doi: 10.3748/wjg.v18.i18.2270
Published online May 14, 2012. doi: 10.3748/wjg.v18.i18.2270
Table 1 Measurement of cell survival rate by the 3-(4,5-dimethylthiazol-2-yl)-2-,5-diphenyltetrazolium bromide assay of pancreatic acinar cells at various time points (mean ± SD)
Table 2 Changes in amylase secretion rate and lactate dehydrogenase leakage rate of pancreatic acinar cells at various time points (mean ± SD)
Index | Group | 0.5 h | 1 h | 4 h | 10 h |
AMS | Non-SDOC group | 7.47 ± 0.67 | 8.97 ± 0.69 | 20.32 ± 2.00 | 24.28 ± 2.47 |
SDOC group | 11.75 ± 2.40a | 17.23 ± 2.43a | 40.88 ± 3.61a | 60.38 ± 4.01a | |
MSCs intervention group | 10.18 ± 1.53 | 14.48 ± 1.74c | 29.33 ± 2.16c | 40.33 ± 4.27c | |
LDH | Non-SDOC group | 3.00 ± 0.63 | 3.47 ± 0.59 | 13.17 ± 2.86 | 23.40 ± 2.55 |
SDOC group | 7.65 ± 1.75a | 12.00 ± 3.17a | 39.02 ± 2.38a | 53.70 ± 6.73a | |
MSCs intervention group | 5.35 ± 1.01c | 8.33 ± 3.08c | 27.67 ± 3.39c | 38.33 ± 3.20c |
Table 3 Comparison of superoxide dismutase and malonaldehyde levels in pancreatic acinar cell culture supernatants at various time points (mean ± SD)
Index | Group | 0.5 h | 1 h | 4 h | 10 h |
Superoxide dismutase (U/mL) | Non-SDOC group | 194.83 ± 26.48 | 185.83 ± 37.79 | 170.00 ± 25.42 | 165.00 ± 31.72 |
SDOC group | 116.17 ± 28.85a | 108.00 ± 41.52a | 102.00 ± 33.45a | 90.67 ± 33.55a | |
MSCs intervention group | 125.50 ± 39.20 | 138.50 ± 42.03 | 147.67 ± 37.25c | 139.00 ± 46.22c | |
Malonaldehyde (μmol/L) | Non-SDOC group | 3.50 ± 5.84 | 4.17 ± 0.75 | 4.33 ± 1.27 | 4.67 ± 1.21 |
SDOC group | 4.40 ± 1.33 | 6.33 ± 1.63a | 7.33 ± 1.21a | 8.00 ± 1.10a | |
MSCs intervention group | 3.97 ± 0.89 | 5.00 ± 1.41 | 5.33 ± 1.63c | 5.83 ± 2.04c |
Table 4 Comparisons of serum amylase levels (U/L) in rats that were or were not treated with mesenchymal stem cells following establishment of an severe acute pancreatitis model (n = 36, mean ± SD)
Table 5 Comparison of serum malonaldehyde, superoxide dismutase levels, interleukin-6, interleukin-10 and tumor necrosis factor-α measured at various time points after injecting either mesenchymal stem cells or saline into rats after establishing an severe acute pancreatitis model (n = 36, mean ± SD)
Index | Group | Post-mesenchymal stem cells or saline injection (h) | ||
6 | 24 | 72 | ||
Malonaldehyde (nmol/mL) | Severe acute pancreatitis group | 4.89 ± 0.97 | 5.20 ± 1.21 | 4.43 ± 0.42 |
Severe acute pancreatitis + mesenchymal stem cells group | 3.68 ± 0.38a | 3.89 ± 0.59a | 3.36 ± 0.98a | |
Superoxide dismutase (U/mL) | Severe acute pancreatitis group | 43.16 ± 6.94 | 48.13 ± 3.93 | 45.83 ± 4.72 |
Severe acute pancreatitis + mesenchymal stem cells group | 48.05 ± 3.83 | 61.29 ± 7.81b | 50.75 ± 7.59 | |
Interleukin-6 (pg/mL) | Severe acute pancreatitis group | 107.70 ± 13.08 | 128.52 ± 8.52 | 134.06 ± 13.12 |
Severe acute pancreatitis + mesenchymal stem cells group | 90.16 ± 9.55a | 107.33 ± 12.13b | 143.24 ± 12.11 | |
Interleukin-10 (pg/mL) | Severe acute pancreatitis group | 31.08 ± 6.64 | 45.02 ± 4.28 | 40.11 ± 8.39 |
Severe acute pancreatitis + mesenchymal stem cells group | 40.84 ± 7.05a | 52.08 ± 5.79a | 41.76 ± 3.37 | |
TNF-α (pg/mL) | Severe acute pancreatitis group | 106.15 ± 9.01 | 132.62 ± 8.64 | 122.42 ± 13.44 |
Severe acute pancreatitis + mesenchymal stem cells group | 91.47 ± 10.00a | 119.47 ± 10.83a | 110.91 ± 9.92 |
Table 6 Comparison of intestinal mucosa injury scores (each slice/score) and proliferating cells of small intestinal mucosa (each slice/number) determined at various time points after injecting either mesenchymal stem cells or saline after establishment of an severe acute pancreatitis model (n = 36, mean ± SD)
Group | Post-MSCs or saline injection (h) | |||||
6 | 24 | 72 | ||||
Intestinal mucosa injury scores | proliferating cells number | Intestinal mucosa injury scores | proliferating cells number | Intestinal mucosa injury scores | proliferating cells number | |
SAP group | 43.33 ± 4.84 | 39.50 ± 5.09 | 52.83 ± 5.27 | 59.67 ± 6.80 | 32.17 ± 4.17 | 81.50 ± 7.89 |
SAP + MSCs group | 36.33 ± 5.72a | 40.83 ± 5.12 | 46.33 ± 2.80a | 68.00 ± 3.22a | 26.67 ± 3.08a | 101.00 ± 11.58b |
- Citation: Tu XH, Song JX, Xue XJ, Guo XW, Ma YX, Chen ZY, Zou ZD, Wang L. Role of bone marrow-derived mesenchymal stem cells in a rat model of severe acute pancreatitis. World J Gastroenterol 2012; 18(18): 2270-2279
- URL: https://www.wjgnet.com/1007-9327/full/v18/i18/2270.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i18.2270