Inhibition of high-mobility group box 1 expression by siRNA in rat hepatic stellate cells

Table 1 Design of small interfering RNA sequences for high-mobility group box 1

Plasmid constructs	Target sequence in mRNA(5’-3’)
HMGB1-1 (shRNAH1)	GCAAATGACTCAATCTGATT
HMGB1-2 (shRNAH2)	AATAGGAAAAGGATATTGCT
HMGB1-3 (shRNAH3)	ACCCGGATGCTTCTGTCAAC

HMGB1: High-mobility group box 1.

Table 2 Effect of high-mobility group box 1 siRNA on the cell cycle

Cell cycle phases(%)	ShRNAH3 group	NC group
G0/G1 phase	58.31% ± 0.48%a	44.25% ± 0.63%
S phase	29.12% ± 1.26%a	41.32% ± 1.58%
G2/M phase	12.57% ± 1.04%	14.53% ± 1.28%

^aP < 0.05 vs negataive controls (NC) group.