Potentially predictive microRNAs of gastric cancer with metastasis to lymph node

Table 1 Clinical-pathological features of 30 biopsies

Clinicopathological variables	n
Gender
Male	16
Female	14
Age
< 60 yr	14
≥ 60 yr	16
Tumor location
Upper third	7
Middle third	16
Lower third	7
Tumor size
< 5 cm	12
≥ 5 cm	18
Histological type
Well and moderately differentiated	11
Poorly differentiated	19
1Depth of invasion
T1	4
T2	8
T3	18
Lymph node involvement
N0	15
N1	8
N2	7
1Tumor node metastasis stage
Stage I	7
Stage II	8
Stage III	11
Stage IV	4

¹According to the tumor node metastasis staging of Union for International Cancer Control.

Table 2 The relative expression of 60 candidate microRNAs (2^-ΔΔCt) in 30 gastric cancer specimens

MiRNA	Median	Percentile		1P value
		25%	75%
Hsa-mir-106b	1.129	0.783	1.982	0.889
Hsa-mir-143	1.231	1.095	1.378	0.779
Hsa-mir-125a-5p	1.255	0.963	1.392	0.889
Hsa-mir-145	1.084	0.931	1.280	1.000
Hsa-mir-25	1.727	1.152	2.103	0.208
Hsa-mir-133b	1.127	0.938	1.314	0.779
Hsa-mir-195	0.989	0.754	1.534	0.889
Hsa-mir-374	1.005	0.800	1.599	0.779
Hsa-mir-451	0.594	0.442	1.636	1.000
Hsa-mir-1	1.228	0.950	2.135	0.327
Hsa-mir-141	0.973	0.663	1.125	0.208
Hsa-mir-200a	0.954	0.757	1.038	0.123
Hsa-mir-29c	0.867	0.664	1.042	0.123
Hsa-mir-29b	1.217	0.964	1.500	0.674
Hsa-mir-30b	0.600	0.542	0.782	0.017
Hsa-mir-26a	1.062	0.787	1.211	0.779
Hsa-mir-26b	1.257	0.838	1.369	0.779
Hsa-mir-144	1.548	1.251	2.118	0.123
Hsa-mir-103	2.103	1.259	2.339	0.050
Hsa-mir-h450b	1.500	1.061	2.601	0.208
Hsa-mir-191	1.770	1.007	2.216	0.161
Hsa-mir-200b	1.314	0.951	1.556	0.401
Hsa-mir-200c	1.151	1.101	1.545	0.161
Hsa-mir-203	1.790	1.464	2.498	0.069
Hsa-mir-429	1.944	1.250	2.028	0.050
Hsa-mir106a	1.279	1.139	1.917	0.069
Hsa-mir-15a	1.782	1.209	1.961	0.093
Hsa-mir-16a	1.145	1.112	1.338	0.017
Hsa-mir-17	1.603	1.396	1.921	0.017
Hsa-mir-155	2.663	1.873	3.557	0.012
Hsa-mir-18a	2.149	2.040	4.214	0.012
Hsa-mir-181b	2.535	2.195	2.635	0.012
Hsa-mir-421	2.141	1.518	2.498	0.012
Hsa-mir-92a	1.848	1.524	2.092	0.012
Hsa-mir-20a	1.596	1.037	2.071	0.036
Hsa-mir-125a-3p	1.729	1.191	2.997	0.036
Hsa-mir-199a-5p	1.645	1.157	2.624	0.069
Hsa-mir-93	2.583	1.250	3.139	0.025
Hsa-mir-222	1.373	1.309	2.488	0.025
Hsa-mir-15b	3.162	1.655	3.821	0.017
Hsa-mir-199a-3p	2.346	1.872	2.779	0.012
Hsa-mir-212	1.543	1.230	2.702	0.050
Hsa-mir-221	2.961	2.440	3.609	0.025
Hsa-mir-147	2.162	1.928	6.019	0.012
Hsa-mir-205	2.008	1.727	15.946	0.017
Hsa-mir-30d	1.614	1.346	2.17	0.017
Hsa-mir-363	1.732	1.612	1.928	0.012
Hsa-mir-23b	1.744	1.339	2.116	0.017
Hsa-mir-214	1.768	1.69	1.902	0.012
Hsa-mir-497	1.520	1.204	2.328	0.017
Hsa-mir-let7c	1.759	1.276	2.300	0.012
Hsa-mir-99a	1.286	1.220	2.734	0.012
Hsa-mir-193b	1.616	1.387	2.554	0.012
Hsa-mir-31	1.749	1.355	3.234	0.012
Hsa-mir-let7b	1.287	1.155	2.071	0.017
Hsa-mir-487b	1.598	1.424	2.855	0.017
Hsa-mir-h450a	1.435	1.116	1.609	0.123
Hsa-mir-18b	1.691	1.413	3.164	0.069
Hsa-mir-19b	1.411	1.248	1.726	0.093
Hsa-mir-19a	1.687	1.209	1.823	0.093
Hsa-mir-let7e	1.195	1.061	1.459	0.123

The relative expression of miRNA (2^-∆∆Ct) in tumor samples, with that in nontumor control samples set at 1.000. ¹P: The Mann-Whitney U test for the different expression of microRNA in different group.

Table 3 Five miRNAs expression in different lymph node metastasis groups

MiRNA	LN Negative (n = 15)	LN Positve (n = 15)	1P value
miR-125a-3p	1.87 (1.27, 2.32)	0.81 (0.49, 1.15)	0.02
miR-133b	1.31 (1.08, 1.59)	0.74 (0.41, 0.97)	0.04
miR-143	1.38 (1.23, 1.57)	0.57 (0.16, 1.03)	0.04
miR-195	1.53 (1.18, 2.52)	0.46 (0.31, 0.64)	0.02
miR-212	2.70 (2.14, 3.16)	1.06 (0.93, 1.18)	0.02

¹P: The Mann-Whitney U test for the different expression of microRNA in different groups. LN: Lymph node.

Table 4 Partial correlation analysis of 5 miRNAs expressions and lymph node metastasis

MicroRNA	Correlation coefficient	P value
miR-125a-3p	-0.451	0.091
miR-133b	-0.014	0.961
miR-143	-0.25	0.369
miR-195	-0.57	0.026
miR-212	-0.616	0.014