Brief Article
Copyright ©2011 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Aug 21, 2011; 17(31): 3645-3651
Published online Aug 21, 2011. doi: 10.3748/wjg.v17.i31.3645
Potentially predictive microRNAs of gastric cancer with metastasis to lymph node
Wen-Yi Wu, Xiang-Yang Xue, Zhe-Jing Chen, Shao-Liang Han, Ying-Peng Huang, Li-Fang Zhang, Guan-Bao Zhu, Xian Shen
Wen-Yi Wu, Zhe-Jing Chen, Shao-Liang Han, Ying-Peng Huang, Guan-Bao Zhu, Xian Shen, Department of General Surgery, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China
Xiang-Yang Xue, Li-Fang Zhang, Department of microbiology, Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China
Author contributions: Wu WY wrote and Xue XY revised the manuscript, Xue XY designed the test, Wu WY and Chen ZJ collected the clinical data, Huang YP collected the samples; Wu WY and Zhang LF did miRNA detection, Han SL and Shen X revised the manuscript, Zhu GB and Shen X helped to analyzed the data and coordinated the whole test.
Supported by Nature Science Foundation of Zhejiang Province No. Y2080978, and Wenzhou Science and Technology Bureau No. H20100028 and No. Y20070067
Correspondence to: Dr. Xian Shen, Department of General Surgery, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China. shenxian5166@yahoo.com.cn
Telephone: +86-577-88069307 Fax: +86-577-88069555
Received: January 26, 2011
Revised: March 24, 2011
Accepted: March 31, 2011
Published online: August 21, 2011
Abstract

AIM: To detect the expression of 60 microRNAs (miRNAs) in gastric cancer tissues and find new predictive biomarkers of gastric cancer with metastasis.

METHODS: The expressions of 60 candidate miRNAs in 30 gastric cancer tissues and paired normal tissues were detected by stem-loop real-time reverse transcription-polymerase chain reaction. After primary screening of miRNAs expression, 5 selected miRNAs were further testified in another 22 paired gastric tissues. Based on the expression level of miRNAs and the status of metastasis to lymph node (LN), receiver-operating-characteristic (ROC) curve were used to evaluate their ability in predicting the status of metastasis to LN.

RESULTS: Thirty-eight miRNAs expressions in gastric cancer tissues were significantly different from those in paired normal tissues (P < 0.01). Among them, 31 miRNAs were found to be up-expressed in cancer tissues and 1 miRNAs were down-expressed ≥ 1.5 fold vs paired normal gastric tissue. Five microRNAs (miR-125a-3p, miR-133b, miR-143, miR-195 and miR-212) were differently expressed between different metastatic groups in 30 gastric cancer biopsies (P < 0.05). Partial correlation analysis showed that hsa-mir-212 and hsa-mir-195 were correlated with the status of metastasis to LN in spite of age, gender, tumor location, tumor size, depth of invasion and cell differentiation. ROC analysis indicated that miR-212 and miR-195 have better sensitivities (84.6% and 69.2%, respectively) and specificities (both 100%) in distinguishing biopsies with metastasis to LN from biopsies without metastasis to LN.

CONCLUSION: miR-212 and miR-195 could be independent biomarkers in predicting the gastric cancer with metastasis to LN.

Keywords: MicroRNA; miR-212; MiR-195; Gastric cancer; Metastasis to lymph node