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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Jul 21, 2009; 15(27): 3411-3416
Published online Jul 21, 2009. doi: 10.3748/wjg.15.3411
Published online Jul 21, 2009. doi: 10.3748/wjg.15.3411
Table 1 Etiologic diagnoses of neonatal intrahepatic cholestasis (NIHC)
Etiology | Number of cases |
Neonatal sepsis | 9 |
Cytomegalovirus | 5 |
Urinary infection tract | 4 |
Syphilis | 2 |
Toxoplasmosis | 2 |
α1-antitripsin deficiency | 5 |
Other metabolic diseases | 5 |
Galactosemia | 4 |
Allagile’s syndrome | 2 |
Byler’s disease | 1 |
Cystic fibrosis | 1 |
Secondary to use of parenteral nutrition | 1 |
Down’s syndrome | 1 |
Panhypopituitarism | 1 |
Idiopathic | 58 |
Total | 101 |
Table 2 Serological results (IgM-ELISA), presence of cytomegalic cells (histological analysis), IHC, and PCR
Methods | Positive | n1 (%) |
IgM-ELISA | 7 | 64 (11) |
Histological analysis | 0 | 84 (0) |
IHC | 1 | 44 (2) |
PCR | 6 | 77 (8) |
Table 3 Clinical and laboratory data of CMV-positive patients by PCR: gender, diagnosis, weight at birth, serology (IgM-ELISA), IHC, and presence of cytomegalic cells
Patients | Gender | Diagnosis | Weight at birth (g) | IgM-ELISA | IHC | Cytomegalic cell |
1 | M | Cystic fibrosis | 3460 | Positive | - | Absent |
2 | F | Idiopathic | 2500 | Negative | Negative | Absent |
3 | M | Byler’s disease | 3320 | Positive | Positive | Absent |
4 | M | Idiopathic | 2700 | Negative | - | Absent |
5 | F | Idiopathic | 1500 | Negative | Negative | Absent |
6 | M | Neonatal sepsis | 2570 | Negative | - | Absent |
Table 4 Results of PCR and serology (IgM-ELISA) and number of patients tested
IgM-ELISA | PCR | ||
+ | - | Total | |
+ | 2 | 5 | 7 |
- | 4 | 40 | 44 |
Total | 6 | 45 | 51 |
Table 5 Results of positive predictive value (PV+), negative predictive value (PV-), accuracy, sensitivity, and specificity between PCR and serology with 95% confidence intervals (CI)
Percent | CI (95%) | ||
Inferior limit | Superior limit | ||
Sensibility | 33.3 | 6.00 | 75.89 |
Specificity | 88.89 | 75.15 | 95.84 |
PV+ | 28.57 | 5.11 | 69.74 |
PV- | 90.91 | 77.42 | 97.05 |
Accuracy | 82.35 | 68.64 | 91.13 |
- Citation: Bellomo-Brandao MA, Andrade PD, Costa SC, Escanhoela CA, Vassallo J, Porta G, De Tommaso AM, Hessel G. Cytomegalovirus frequency in neonatal intrahepatic cholestasis determined by serology, histology, immunohistochemistry and PCR. World J Gastroenterol 2009; 15(27): 3411-3416
- URL: https://www.wjgnet.com/1007-9327/full/v15/i27/3411.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.3411