Protective effect of Radix Astragali injection on immune organs of rats with obstructive jaundice and its mechanism

Table 1 Comparison of serum TNF-α levels in different groups (ng/L, mean ± SD)

Group	7 d	14 d	21 d	28 d
Sham-operation group	12.89 ± 1.63	12.25 ± 3.37	14.21 ± 3.24	15.61 ± 4.84
Model control group	29.55 ± 18.02b	34.10 ± 8.94b	31.52 ± 10.95b	57.66 ± 12.99b
Treatment group	27.62 ± 12.61ab	27.20 ± 9.34b	24.61 ± 9.09ab	39.01 ± 9.62b

^aP < 0.05 vs model control group;

^bP < 0.01 vs sham-operation group.

Table 2 Pathological severity scoring standards for spleen

Score standards	Observation indexes
0	Normal
1	Necrosis of follicle center
2	Blood sinus dilation or arteriolar sclerosis
3	Necrosis of follicle center, blood sinus dilation and arteriolar sclerosis

Table 3 Comparison of different pathological indexes for spleen, M (Q_R)

Index	Time (d)	Sham-operation group	Model control group	Treatment group
Pathological severity score	7	0.0 (0.0)	0.0 (0.0)	0.0 (0.0)c
	14	0.0 (1.0)c	0.0 (2.0)	0.0 (0.0)c
	21	0.0 (0.0)	1.0 (2.0)	0.0 (0.0)
	28	0.0 (0.0)	0.0 (1.0)	0.0 (0.0)
Product staining intensity and positive rate of Bax	7	0.0 (0.0)c	1.0 (2.0)	0.5 (1.0)a
	14	0.0 (1.0)	1.0 (2.0)	0.0 (0.0)c
	21	0.0 (0.0)c	1.0 (2.0)	0.0 (2.0)
	28	0.0 (0.0)c	2.0 (1.5)	0.0 (1.0)c
Apoptosis index	7	0.0 (0.0)	0.0 (0.0)	0.0 (0.0)
	14	0.0 (0.0)	0.0 (0.01)	0.0 (0.0)c
	21	0.0 (0.0)c	0.0 (0.01)	0.0 (0.0)
	28	0.0 (0.0)c	0.01 (0.02)	0.0 (0.0)
Product staining intensity and positive rate of NF-κB	7	0.0 (0.0)	0.0 (1.0)	0.0 (0.0)
	14	0.0 (1.0)	0.0 (2.0)	0.0 (0.0)
	21	0.0 (0.0)	0.0 (0.0)	0.0 (0.0)
	28	0.0 (0.0)	0.0 (0.0)	0.0 (0.0)

^aP < 0.05 vs sham-operation group;

^cP < 0.05 vs model control group.

Table 4 Comparison of pathological severity scores for lymph nodes

Groups	7 d	14 d	21 d	28 d
Sham-operation group	0.0 (2.0)c	0.0 (1.0)c	0.0 (1.0)c	0.0 (1.0)
Model control group	1.0 (2.0)	1.0 (1.0)	1.0 (2.0)	2.0 (1.0)
Treatment group	0.0 (0.0)a	0.0 (1.0)	0.0 (1.0)c	1.0 (0.0)c

^aP < 0.05 vs sham-operation group;

^cP < 0.05 vs model control group.

Table 5 Comparison of different pathological indexes for thymus, M (Q_R)

Index	Time (d)	Sham-operation group	Model control group	Treatment group
Product staining intensity and positive rate of Bax	7	0.0 (0.0)c	1.0 (2.0)	0.5 (1.0)a
	14	0.0 (1.0)	1.0 (2.0)	0.0 (0.0)c
	21	0.0 (0.0)c	1.0 (2.0)	0.0 (2.0)
	28	0.0 (0.0)c	2.0 (1.5)	0.0 (1.0)c
Apoptosis index	7	0.0 (0.0)	0.0 (0.0)	0.0 (0.0)
	14	0.0 (0.0)	0.0 (0.01)	0.0 (0.0)c
	21	0.0 (0.0)c	0.0 (0.01)	0.0 (0.0)c
	28	0.0 (0.0)c	0.01 (0.02)	0.0 (0.0)
Product of the staining intensity and positive rate of NF-κB	7	0.0 (0.0)	0.0 (2.0)	0.0 (0.0)
	14	0.0 (4.0)	2.0 (4.0)	0.0 (2.0)
	21	0.0 (2.0)	0.0 (2.0)	0.0 (0.0)
	28	0.0 (1.0)	0.0 (3.0)	0.0 (2.0)

^aP < 0.05 vs sham-operating group;

^cP < 0.05 vs model control group.