TSPAN1 protein expression: A significant prognostic indicator for patients with colorectal adenocarcinoma

Table 1 Correlation of clinicopathological parameters with TSPAN1 expression

Parameters	Cases	TSPAN1 expression intensity				P
		-	+	++	+++
Gender
Male	50	9	14	21	6	0.472
Female	38	10	12	10	6
Tumor size (cm)
< 4.0	35	10	10	10	5	0.469
> 4.0	53	9	16	21	7
Type
Cauliflower/polyp	48	9	14	16	9	0.595
Ulcer/infiltration	40	10	12	15	3
Location
Rectum	41	9	11	17	4	0.595
Colon	47	10	15	14	8
Grade
Well	14	6	6	2	0	0.001
Moderate	39	9	14	14	2
Poor	35	4	6	15	10
PCNA
+	43	14	14	13	2	0.015
++/+++	45	5	12	18	10
Lymph node metastasis
No	55	16	20	14	5	0.008
Yes	33	3	6	17	7
TNM stage
I	29	11	9	7	2	0.002
II	26	5	11	7	3
III-IV	33	3	6	17	7
Vascular invasion
No	62	14	21	20	7	0.424
Yes	26	5	5	11	5
Perineural invasion
No	67	16	22	22	7	0.235
Yes	21	3	4	9	5
Desmoplasia
No	55	10	16	20	9	0.647
Yes	33	9	10	11	3

Table 2 Univariate analysis by Log-rank test

Parameters	5-yr survival rate (%)	Log-rank test
		χ2	P
TSPAN1 expression
-	66.67 (6/9)	15.48	0.0015
+	71.4 (5/7)
++	35.3 (6/17)
+++	11.1 (1/9)
Grade
Well	87.5 (7/8)	6.91	0.0316
Moderate	37.5 (6/16)
Poor	27.8 (5/18)
Node status
No	71.6 (12/17)	15.67	0.0001
Yes	24.0 (6/25)
PCNA expression
+	63.1 (12/19)	9.05	0.0026
++-+++	26.1 (6/23)
TNM stages
I	83.3 (10/12)	16.20	0.0030
II	62.5 (5/8)
III-IV	13.6 (3/22)
Vascular invasion
No	46.4 (13/28)	1.39	0.2377
Yes	37.7 (5/14)
Perineural invasion
No	44.7 (14/32)	0.77	0.3795
Yes	40.0 (4/10)
Desmoplasia
No	33.3 (3/9)	0.02	0.8829
Yes	42.4 (15/33)

Table 3 Multivariate analysis in Cox proportional hazard model

Variable	Multivariate analysis				Pvalue
	HR	SD	Z	95% CI
TSPAN1 expression	0.755	0.231	3.27	0.302-1.208	0.001
Grade	0.798	0.318	2.51	0.175-1.421	0.012
Node status	1.779	0.509	3.49	0.781-2.778	0.000
PCNA expression	1.325	0.475	2.79	0.394-2.256	0.005
TNM stages	1.159	0.341	3.39	0.490-1.829	0.001
Vescular invasion	0.491	0.423	1.16	0.338-1.320	0.246
Perineural invasion	0.409	0.473	0.87	0.517-1.336	0.386
Desmoplasia	0.061	0.415	0.15	0.752-0.873	0.884