Ultrastructural changes in hepatocytes after taurine treatment in CCl4 induced liver injury

Table 1 Ultrastructural scoring system used in experimental liver fibrosis[19-21]

Ultrastructure	Assessment	Score
Mitochondria	Normal	0
	Prominent cristae	1
	Edematous mitochondrion	2
	Collection of amorphous material	3
rER	Normal	0
	Dilatation	1
	Irregular lamellar organization	2
	Presence of focal breaks	3
sER	Normal	0
	Dilatation	1
	Vacuolization	2
	Presence of large degenerated areas, myelin figures	3
Nucleus	Normal	0
	Irregular chromatin distribution (margination, clumping)	1
	Increased heterochromatin	2
	Degenerated nucleus	3

rER: Rough endoplasmic reticulum; sER: Smooth endoplasmic reticulum.

Table 2 Histopathologic scoring and organelle injury scoresof Group 1 and Group 2 (mean ± SD)

	Group 1	Group 2	P
Histopathologic scoring
Necrosis	1.20 ± 0.15	2.27 ± 0.18	< 0.0011
Inflammation	1.40 ± 0.13	2.00 ± 0.17	< 0.031
Fat accumulation	1.27 ± 0.12	2.07 ± 0.21	< 0.011
Fibrosis	1.40 ± 0.16	2.27 ± 0.18	< 0.0051
Total	5.20 ± 0.38	8.60 ± 0.29	< 0.0011
Organelle injury scores
Mitochondrion	45.7 ± 1.3	98.9 ± 2.1	< 0.0012
Rough ER	22.1 ± 0.8	41.6 ± 2.1	< 0.0012
Smooth ER	20.4 ± 0.8	43.3 ± 1.6	< 0.0012
Nucleus	17.9 ± 1.2	33.3 ± 0.9	< 0.0012
Total	106 ± 4	217 ± 6	< 0.0012

¹Mann-Whitney U test;

²Student’s t-test. ER: Endoplasmic reticulum.