Effect of BN52021 on NFκ-Bp65 expression in pancreatic tissues of rats with severe acute pancreatitis

Table 1 Scoring standard of pathological change for pancreatic tissue of rats with SAP

Pathological grading	Pathological change	Scores
Edema	Inter-lobule local edema, widened pleura	1
	Inter-lobule diffuse edema, widened intra-lobule clearance	2
	Increased intra-lobule clearance, alveolus swollen, and separated	3
Inflammatory	White cells < 20/visual field under high-power microscope	1
Cell infiltration	White cells 20-50/visual field under high-power microscope	2
	White cells > 30/visual field under high-power microscope, or micro-abscess occurs	3
Hemorrhage	Parenchymal hemorrhage < 20%	1
	Parenchymal hemorrhage 20%-50%	2
	Parenchymal hemorrhage > 50%	3
Necrosis	Necrosis area < 20%	1
	Necrosis area 20%-50%	2
	Necrosis area > 50%	3

Table 2 PCR primer series, position and segment length of NF-κBp65 and β-actin.

Gene	Primer series (5'-3')	Gene No.	Position	Length
NF-κBp65	GAAGAAGCGAGACCTGGAG	NM_199267	424-442	398 bp
	TCCGGAACACAATGGCCAC		821-803
β-actin	TCCTAGCACCATGAA GATC	NM_031144	1044-1062	190 bp
	AAACGCAGCTCAGTAACAG		1233-1215

Table 3 Level of serum amylase at each time point in each group after operation (U/L) n = 10, mean ± SD

Groups	Time points
	1 h	2 h	3 h	6 h	12 h	24 h
NC	1835.6	1491.5	1530.4	1400.2	2153.2	337
	± 613.2	± 507.0	± 247.0	± 447.5	± 236.3	± 43.7
SAP	2560.5	2810.5	4799.3	4919.7	3486.3	2283
	± 121.5a	± 147.2a	± 107.0a	± 139.6a	± 181.8a	± 127.0a
BN	2214.5	3331.7	4185	3784.7	3454	1360.4
	± 109.1b	± 196.4b	± 147.8bc	± 124.1bc	± 264.1b	± 161.4bc

^aP < 0.05,

^bP < 0.05 vs NC group;

^cP < 0.05 vs SAP group.

Table 4 Scores of pancreatic tissues at each time point in each group after operation n = 10, mean ± SD

Groups	Time points
	1 h	2 h	3 h	6 h	12 h	24 h
NC	0.12 ± 0.05	0.11 ± 0.06	0.12 ± 0.05	0.13 ± 0.04	0.12 ± 0.06	0.13 ± 0.05
SAP	4.82 ± 0.35a	7.65 ± 0.40a	8.85 ± 0.39a	9.15 ± 0.55a	10.10 ± 0.65a	11.75 ± 0.25a
BN	5.55 ± 0.30b	6.65 ± 0.42b	5.95 ± 0.19bc	5.55 ± 0.36bc	6.72 ± 0.30bc	9.95 ± 0.58b

^aP < 0.05,

^bP < 0.05 vs NC group;

^cP < 0.05 vs SAP group.

Table 5 Absorbance values of NF-κBp65 mRNA in pancreatic tissue at each time in each group after operation n =10, mean ± SD

Groups	Time points
	1 h	2 h	3 h	6 h	12 h	24 h
NC	220.01 ± 94.51	186.30 ± 51.52	167.19 ± 51.55	114.91 ± 55.96	123.91 ± 54.78	131.08 ± 66.84
SAP	340.47 ± 119.14	269.03 ± 66.03a	229.45 ± 50.34a	197.07 ± 96.02	307.42 ± 100.62a	376.97 ± 0.22a
BN	470.80 ± 122.78bc	268.70 ± 87.64b	267.95 ± 49.66b	277.64 ± 83.37b	337.21 ± 103.02b	340.24 ± 0.44b

^aP < 0.05,

^bP < 0.05 vs NC group;

^cP < 0.05 vs SAP group.

Table 6 Absorbance values of NF-κBp65 protein in pancreatic tissue at each time point in each group after operation. n = 10, mean ± SD

Groups	Time points
	1 h	2 h	3 h	6 h	12 h	24 h
NC	0.05 ± 0.00	0.09 ± 0.01	0.07 ± 0.01	0.05 ± 0.01	0.06 ± 0.01	0.10 ± 0.02
SAP	1.43 ± 0.06a	0.80 ± 0.05d	1.58 ± 0.02a	1.38 ± 0.03a	0.87 ± 0.09d	0.90 ± 0.05d
BN	1.04 ± 0.02bc	0.72 ± 0.02b	1.01 ± 0.04bc	0.95 ± 0.03bc	0.75 ± 0.02b	0.76 ± 0.01b

^aP < 0.05,

^bP < 0.05,

^dP < 0.05 vs NC group;

^cP < 0.05 vs SAP group.