Relationship between vascular invasion and microvessel density and micrometastasis

Table 1 Oligonucleotide primers

cDNA	Primer	Sequence	Product length (bp)
CK20	Outer sense	5'-GAGGTTCAAC TAACGGAGCT-3'	253
	Outer antisense	5'-TCTCTCTTCCA GGGTGCTTA-3'
	Inner sense	5'-GCCTTGAGATA GAACTCCAG-3'
	Inner antisense	5'-ACGTCTTCTCC TTCCAGAAG-3'
GAPDH	Sense	5'-CAGGGCTGCTT TTAACTCTG-3'	385
	Antisense	5'-CTGTTGTCGGAG TTCTAGTAG-3'

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Table 2 Comparison between HE and immunohistochemical staining

	Vascular invasion		χ2	P
	(+)	(-)
HE staining	9	37	19.087	< 0.05
Immunohistochemical staining	17	29

McNemar's test for correlated proportions, χ² = 8.003, P < 0.05 vs immunohistochemical staining.

Table 3 Comparative data on vascular invasion

	n	VI(+)	VI(-)	χ2	P
CK20 mRNA
Positive	21	12	9	6.758	< 0.05
Negative	25	5	20
Age (yr)
< 50	14	4	10	0.607	> 0.05
≥ 50	32	13	19
Size (cm)
< 5	31	12	19	0.125	> 0.05
≥ 5	15	5	10
Differentiated
Well	11	2	9	2.351	> 0.05
Moderately	20	8	12
Poorly	15	7	8
Serosa invasion
Negative	14	2	12	4.440	< 0.05
Positive	32	15	17
Lymph node metastasis
Negative	18	3	15	5.225	< 0.05
Positive	28	14	14
Distant metastasis
Negative	38	9	29	16.520	< 0.05
Positive	8	8

Statistical analysis by chi-square test. VI: Vascular invasion.

Table 4 Average number of microvessels of tissue in VI positive and negative patients

	n	MVD	t	P
VI
Positive	17	29.2 ± 3.3	2.987	< 0.05
Negative	29	25.4 ± 4.7

Statistical analysis of independent samples by t test. VI: Vascular invasion; MVD: Microvessel density.