Rapid Communication
Copyright ©2007 Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 14, 2007; 13(46): 6269-6273
Published online Dec 14, 2007. doi: 10.3748/wjg.v13.i46.6269
Relationship between vascular invasion and microvessel density and micrometastasis
Yan-Dong Wang, Pei Wu, Jia-Ding Mao, He Huang, Fan Zhang
Yan-Dong Wang, Department of Surgery, Wannan Medical College, Wuhu 241001, Anhui Province, China
Pei Wu, Jia-Ding Mao, He Huang, Department of Surgery, Affiliated Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China
Fan Zhang, Department of Pathology, Affiliated Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Pei Wu, Department of Surgery, Affiliated Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China. wp5708@sohu.com
Telephone: +86-13637134296
Received: April 17, 2007
Revised: September 12, 2007
Accepted: October 26, 2007
Published online: December 14, 2007
Abstract

AIM: To evaluate the relationship between vascular invasion and microvessel density (MVD) of tissue and micrometastasis in blood.

METHODS: Vascular invasion was detected by both hematoxylin and eosin staining and immunohistochemiscal staining. Blood samples were collected from 17 patients with vascular invasion and 29 patients without vascular invasion and examined for cytokeratin20 (CK20) expression by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Microvessel density of tissue samples was also determined by immunohistochemistry using antibodies to CD105.

RESULTS: CK20 was detected in 12 of the 17 patients with vascular invasion and in 9 of the 29 patients without vascular invasion. Positive RT-PCR was significantly correlated with vascular invasion (70.6% vs 30.0%, P < 0.05). The average MVD was significantly higher in patients with positive vascular invasion than in patients with negative vascular invasion (29.2 ± 3.3 vs 25.4 ± 4.7, P < 0.05). The vascular invasion detected with hematoxylin-eosin staining was less than that with immunohistochemical staining. There was a significant difference between the two staining methods (19.6% vs 36.9%, P < 0.05).

CONCLUSION: Positive CK20 RT-PCR, depth of tumor invasion, lymph node status, metastasis and MVD are significantly correlated with vascular invasion. Immunohistochemical staining is more sensitive than hematoxylin-eosin staining for detecting vascular invasion.

Keywords: Vascular invasion; Reverse transcriptase-polymerase chain reaction; Microvessel density; Micrometastasis