Experimental small bowel preservation using Polysol: A new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?

Table 1 Composition of UW, celsior, HTK and polysol

Components	UW	Celsior	HTK	Polysol
Colloid (g/L)	HES (250 kDa), 50	-	-	PEG (35 kDa), 20
Na/K ratio (mmol/L)	27/125	100/15	10/15	135/5
Buffers (mmol/L)	H2PO4-, 25	Histidine, 30	Histidine, 180	Histidine, 6.3
				H2PO4-, 21.74
				HEPES, 20
Antioxidants (mmol/L)	Allopurinol, 1	Glutathion, 3	-	Allopurinol, 1.2
	Glutathion, 3			Glutathion, 3
				Alpha-tocopherol, 5 × 10-5
				Ascorbic acid, 0.11
Nutrients (mmol/L)	-	-	Glucose, 28	Glucose, 11.1
				Adenine, 5
				Sodium pyruvate, 0.23
Impermeants (mmol/L)	Lactobionate, 100	Lactobionate, 80		Raffinose, 3
	Raffinose, 30			Trehalose, 5.3
				Na+-Gluconate, 74.99
				K+-Gluconate, 20
Amino acids (mmol/L)	-	Glutamic acid, 20	Tryptophan, 2	various1, 11
Vitamins (mmol/L)	-	-	-	various2, 0.17
Ca2+/Mg2+ (mmol/L)	-	0.25/13	0.015/4	2/4
Others (mmol/L)	Adenosine, 5	Mannitol, 60	Mannitol, 30 Ketoglutarate, 1	Adenosine, 5
pH	7.4	7.3	7.2	7.4
Osmolarity (mOsm/L)	320	320	310	320
Viscosity at 5°C (cP)	5.7	1.3	1.8	1.8

UW: University of Wisconsin solution; HTK: histidine-tryptophan-ketoglutarate solution; HES: hydroxyethyl starch; PEG: polyethylene glycols; cP: centi-Poise.

¹The following amino acids (mmol/L) are supplemented in Polysol: alanine (1.01), arginine (1.18), asparagines (0.08), aspartic acid (0.23), cystine (0.33), cystine (0.25), glutamic acid (0.34), glutamine (0.002), glycine (0.67), isoleucine (0.38), leucine (0.57), lysine (0.48), methionine (0.30), ornithine (2.00), phenylalanine (0.30), proline (0.78), serine (0.29), threonine (0.34), tryptophan (0.88), tyrosine (0.19), and valine (0.43).

²The following vitamins (mmol/L) are supplemented in Polysol: ascorbic acid (0.11), biotin (0.21), Ca-pantothenate (0.004), choline chloride (0.01), inositol (0.07), ergocalciferol (3 × 10^-4), folic acid (0.002), menadione (4 × 10^-5), nicotinamide (0.01), nicotinic acid (0.004), pyridoxal (0.005), riboflavin (0.003), thiamine (0.03), vitamin A (3 × 10^-4), vitamin B12 (1 × 10^-4) and vitamin E (5 × 10^-5).