Basic Research
Copyright ©2007 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jul 21, 2007; 13(27): 3684-3691
Published online Jul 21, 2007. doi: 10.3748/wjg.v13.i27.3684
Experimental small bowel preservation using Polysol: A new alternative to University of Wisconsin solution, Celsior and histidine-tryptophan-ketoglutarate solution?
Lai Wei, Koichiro Hata, Benedict Marie Doorschodt, Reinhard Büttner, Thomas Minor, René H Tolba
Lai Wei, Koichiro Hata, René H Tolba, House of Experimental Therapy, University of Bonn, 53105 Bonn, Germany
Lai Wei, Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Benedict Marie Doorschodt, Surgical Laboratory, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands
Reinhard Büttner, Institute of Pathology, University of Bonn, 53105 Bonn, Germany
Thomas Minor, Surgical Research Division, Department of Surgery, University of Bonn, 53105 Bonn, Germany
Author contributions: All authors contributed equally to the work.
Supported by the BONFOR-Grant and the DFG grant, No. TO 542/1-1 to RHT
Correspondence to: Dr. René H Tolba, House of Experimental Therapy, University of Bonn, Sigmund Freud Str. 25, 53105, Bonn, Germany. rtolba@uni-bonn.de
Telephone: +49-228-28719120 Fax: +49-228-28711657
Received: April 6, 2007
Revised: April 23, 2007
Accepted: April 26, 2007
Published online: July 21, 2007
Abstract

AIM: To evaluate the potential of Polysol, a newly developed preservation solution, in cold storage of small bowel grafts, compared with the current standards, University of Wisconsin solution (UW), Celsior and histidine-tryptophan-ketoglutarate solution (HTK).

METHODS: Male Wistar rats were used as donors. Small bowels were retrieved, flushed and then stored in the respective 4 solutions for 18 h at 4°C. Functional integrity of the grafts was evaluated by isolated reperfusion with oxygenated Krebs-Henseleit buffer at 37°C for 30 min in all 4 groups.

RESULTS: Polysol preservation exhibited the highest tissue ATP concentration and the lowest release of LDH. Malondialdehyde, an index for tissue lipid peroxidation, was also the lowest in Polysol. Tissue oxygen consumption was significantly higher in Polysol than in the others. Of interest, UW-storage promoted 10-fold higher apoptosis than in the others. Moreover, electron microscopy revealed that the mucosal villi/micro-villi formation and the cell organelles, including mitochondria, were both significantly better preserved in Polysol, while deleterious alterations were apparent in the others, most notably in UW. Although Celsior and HTK exhibited the better trend of results than UW in some parameters, but could not reach the over-all superiority to UW.

CONCLUSION: Cold storage using Polysol resulted in significantly better integrity and function of small bowel grafts than UW. Hence, Polysol may be a novel alternative for the small bowel preservation.

Keywords: Small bowel transplantation; Ischemia/reperfusion; Cold storage; Polysol; University of Wisconsin solution; Histidine-tryptophan-ketoglutarate solution; Celsior