Multiple pathogenic factor-induced complications of cirrhosis in rats: A new model of hepatopulmonary syndrome with intestinal endotoxemia

Table 1 Changes of hepatic function in rats (mean ± SD, n = 6)

Group	ALT(U/L)	AST(U/L)	TB(mmol/L)	Hyp(mg/g)	PVP(H2O cm)
Control	37.24 ± 6.96	68.68 ± 13.36	8.15 ± 1.70	0.60 ± 0.07	10.31 ± 1.16
Cirrhosis	84.12 ± 13.14a	186.23 ± 31.83a	40.25 ± 3.21a	2.08 ± 0.09a	18.38 ± 2.53a

^aP < 0.01 vs normal control.

Table 2 Analysis of blood gas in rats (mean ± SD)

Groups	n	pH	PaCO2 (mmHg)	PaO2 (mmHg)	O2 cont (mL/dL)	AaDO2 (mmHg)
Normal control	8	7.38 ± 0.03	33.94 ± 4.21	94.71 ± 13.72	20.40 ± 0.32	3.44 ± 3.26
Normal + LPS	7	7.31 ± 0.02	28.13 ± 2.56	117.51 ± 22.30	20.63 ± 0.34	4.60 ± 2.24
Cirrhosis	7	7.34 ± 0.14	28.76 ± 5.57	88.87 ± 28.46	19.80 ± 1.1a	9.90 ± 2.08c
Cirrhosis + LPS	6	7.32 ± 0.04	24.98 ± 8.69	112.55 ± 24.02	20.58 ± 0.27	18.48 ± 7.86ce
Cirrhosis + Glycine + LPS	6	7.31 ± 0.10	33.25 ± 5.10	110.40 ± 12.71	20.60 ± 0.14	-

^aP < 0.05 vs other groups;

^cP < 0.05 vs normal control;

^eP < 0.05 vs cirrhosis group.

Table 3 Analysis of correlation

PAIRS	n	r	P value
Plasma LPS-Plasma TNF-α	30	0.443	< 0.01
Plasma LPS-O2 content	30	0.47	< 0.05
Plasma LPS-Tc99MAA	20	0.505	< 0.05
Plasma TNF-α-Tc99MAA	20	0.475	< 0.05

Table 4 Changes of pulmonary coefficient in rats (mean ± SD, n = 6)

Groups	Pulmonary coefficient
Normal control	7.3489 ± 0.8225
Normal + LPS	7.9728 ± 0.9203
Cirrhosis	7.5992 ± 0.7960
Cirrhosis + LPS	10.8895 ± 4.6496a
Cirrhosis + Glycine + LPS	8.5914 ± 0.1875

^aP < 0.01 vs normal control.

Table 5 Bacterial translocation of mesenteric lymph node in rats (n = 5)

Groups	Minor clony	Major clony
Normal control	5	0
Cirrhosis	2	3

Gram reaction: gram-negative predominantly.

Table 6 Levels of LPS and TNF-α in plasma in rats (mean ±SD, n = 6)

Groups	LPS (EU/mL)	TNF-α (ng/mL)
Normal control	0.0571 ± 0.0104	0.6629 ± 0.1620
Normal + LPS	0.5550 ± 0.0699a	0.9344 ± 0.2819e
Cirrhosis	0.1655 ± 0.0410a	1.0588 ± 0.1337e
Cirrhosis + LPS	0.5269 ± 0.0659a	1.5571 ± 0.1785e
Cirrhosis + Glycine + LPS	0.3144 ± 0.0649ac	1.2560 ± 0.1356eg

LPS:

^aP < 0.05 vs normal control group;

^cP < 0.05 vs cirrhosis + LPS group; TNF-α:

^eP < 0.05 vs normal control group;

^gP < 0.05 vs cirrhosis + LPS group.