Protein profile of human hepatocarcinoma cell line SMMC-7721: Identification and functional analysis

Table 1 Information of the identified proteins in human hepatocarcinoma cell line SMMC-7721

No.	NCBI/SWISS-PROT accession No.	Protein description	Theoretical pI/Mr (kDa)	Experimental pI/Mr (kDa)	Profound score	Normal volume
159	P38646	HSP70, heat shock 70-ku protein 9B precursor	5.9/73.7	5.4/71.0	2.37	1.228
149	P38646	HSP70, heat shock 70-ku protein 9B precursor	5.9/73.7	5.4/71.8	2.30	-
259	P10809	Chaperonin CH60,	5.7/61.2	5.3/58.7	2.33	0.271
		60-ku heat-shock protein, mitochondrial precursor
136	P11021	GRP78, Bip, 78-ku glucose-regulated protein precursor	5.2/71.0	5.1/75.4	2.37	-
275	P27797	Calreticulin precursor	4.3/48.2	4.4/56.0	2.35	0.383
757	P30040	Endoplasmic reticulum protein ERp29 precursor (ERp31) (ERp28)	6.8/29.0	6.0/29.3	2.26	0.374
278	P30101	Protein disulfide isomerase ER60 precursor	6.0/57.1	5.7/55.6	2.42	0.958
265	P07237	Protein disulfide isomerase precursor	4.8/57.0	4.8/57.7	2.41	0.734
		(Prolyl 4-hydroxylase, beta subunit)
789	Q13162	Peroxiredoxin-4 (Prx-IV) (Thioredoxin peroxidase AO372) (Thioredoxin-dependent peroxide reductase A0372) (antioxidant enzyme AOE372)	5.9/30.7	5.6/28.5	2.38	0.785
849	32483377	(NP_054817.2) Peroxiredoxin 3 isoform b; thioredoxin-dependent peroxide reductase precursor	7.1/26.1	6.0/26.5	1.73	1.141
645	P21796	Voltage-dependent anion channel protein I (VDAC1) (plasmalemmalporin) (outer mitochondrial membrane protein porin) (PORIN 31NL) (porin 31HM)	8.8/30.8	9.2/34.4	2.23	1.572
372	P31930	Ubiquinol-cytochrome C reductase complex core protein I	5.9/53.3	5.5/45.5	2.29	0.121
699	Q13011	Peroxisomal enoyl-CoA hydratase	6.6/36.1	6.0/32.3	2.14	0.927
319	P06576	ATP synthase beta chain, mitochondrial precursor	5.3/56.6	5.1/51.1	2.39	0.222
774	P30084	Mitochondrial short-chain enoyl-coenzyme A hydratase 1	6.9/32.0	5.9/29.0	2.26	0.773
295	P55809	Probable 3-oxoacid CoA transferase 1 precursor	7.2/56.6	6.0/53.5	1.65	0.449
108	Q16891	Mitochondrial inner membrane protein (Mitofilin) (p87/89)	6.1/84.0	5.8/81.8	2.24	0.410
714	P35232	Prohibitin	5.6/29.9	5.5/31.1	2.18	1.923
397	P08670	Vimentin	4.8/41.7	4.8/43.2	2.19	-
283	P07437	Tubulin beta-2 chain	4.7/50.2	5.0/55.0	2.37	-
153	P02545	Lamin A/C isoform 1 precursor	6.6/74.0	6.2/71.6	2.30	0.297
150	P02545	Lamin A/C isoform 2	6.4/65.1	6.1/71.8	2.28	0.444
246	P02545	Lamin A	6.4/57.7	6.2/60.4	1.80	0.16
415	P02570	Actin cytoplasmic 1	5.3/41.7	5.3/42.4	2.39	-
416	P02570	Actin cytoplasmic 1	5.3/41.7	5.4/42.6	2.23	-
417	P02570	Actin cytoplasmic 1	5.3/41.7	5.4/42.7	2.18	-