Differences in oxidative stress dependence between gastric adenocarcinoma subtypes

Table 1 Correlation of staining with clinicopathologic data

Clinicopathologic featuresa	iNOSbNo. of casesTotal positive (%)	NTYRbNo. of casesTotal positive (%)	8OH-dGbNo. of casesTotal positive (%)
Infiltrative cancer subtypes	85 35 (41)	65 40 (62)	82 41 (50)
TC	54 291 (541)	43 312 ( 722)	54 28 (52)
DC	17 31 ( 181)	12 6 (50)	14 7 (50)
PolyC	14 31 (211)	10 32 ( 302)	14 6 (43)
Intraepithelial cancer
Periinfiltrative cancer	5 3 (60)	6 4 (67)	6 4 (67)
Without infiltrative cancer	7 1 (14)	7 6 (86)	10 5 (50)
Topography of infiltrative cancer
Cardia	10 4 (40)	7 3 (43)	11 5 (45)
Gastric stump	12 6 (50)	9 5 (56)	8 3 (38)
Antrum with angulus+pylorus	45 18 (40)	35 22 (63)	41 22 (54)
Body	14 7 (50)	11 9 (82)	15 11 (73)
Peritumorous mucosa
Adjacent: N + no IM G	15 5 (33)	23 9 (39)	30 10 (33)
Adjacent: atrophy IM	23 14 (61)	21 10 (48)	26 18 (69)
At distance: N + no IM G	3 1 (33)	6 1 (17)	5 2 (40)
At distance: atrophy IM	7 4 (57)	7 5 (71)	13 9 (69)
Adjacent+distant: N + no IM G`	18 6 (33)	29 10 (34)	35 123 (343)
Adjacent+distant: atrophy IM	30 18 (60)	28 15 (54)	39 27 (69)
Precancerous lesions
Low grade dysplasia	13 9 (69)	14 11 (79)	17 143 (823)
Atrophy IM without cancer	10 8 (80)	7 7 (100)	11 6 (55)
Atrophy IM with + without cancer	40 26 (65)	35 22 (63)	50 333 (663)
H pylori infection
Negative	68 27 (40)	54 35 (65)	77 41 (53)
Positive	18 7 (39)	13 7 (54)	16 9 (56)
Sex
Male	60 28 (47)	50 32 (64)	66 36 (55)
Female	30 8 (27)	29 20 (69)	35 18 (51)

(a) TC: Intestinal or tubular carcinoma; DC: Diffuse carcinoma; PolyC: Mixed polymorphous carcinoma; N: Normal; IM: Intestinal metaplasia; G: Gastritis. (b) iNOS: Inducible nitric oxide synthase; NTYR: Nitrotyrosine; 8OH-dG: 8-hydroxy-2’-deoxyguanosine.

¹TC vs DC P = 0.008; TC vs PolyC, P = 0.04; TC vs DC+PolyC, P = 0.04;

²TC vs PolyC. P = 0.03;

³non-metaplastic mucosa atrophic metaplastic mucosa low-grade dysplasia, P = 0.005.

Table 2 Correlation of staining with pTNM pathological classification^a

Pathological classificationb	iNOScNo. of casesTotal positive (%)	NTYRcNo. of casesTotal positive (%)	8OH-dGcNo. of casesTotal positive (%)
pTis	7 1 (14)	7 6 (86)	10 5 (50)
pT1	18 7 (39)	16 12 (75)	16 10 (63)
TC	13 7 (54)	12 9 (75)	12 8 (67)
DC	3 0 (0)	2 2 (100)	2 2 (100)
PolyC	2 0 (0)	2 1 (50)	2 0 (0)
pT2	7 3 (43)	8 6 (75)	8 4 (50)
TC	5 2 (40)	6 5 (83)	6 3 (50)
DC	0 0 (0)	0 0 (0)	0 0 (0)
PolyC	2 1 (50)	2 1 (50)	2 1 (50)
pT3/T4	22 9 (41)	15 8 (53)	21 10 (48)
TC	12 7 (58)	8 6 (75)	12 6 (50)
DC	5 1 (20)	3 1 (33)	4 2 (50)
PolyC	5 1 (20)	4 1 (25)	5 2 (40)
pN0	20 10 (50)	20 14 (70)	20 11 (55)
TC	16 8 (50)	16 13 (81)	16 10 (63)
DC	1 1 (100)	1 0 (0)	1 1 (100)
PolyC	3 1 (33)	3 1 (33)	3 0 (0)
pN+	24 8 (33)	17 9 (53)	22 11 (50)
TC	10 6 (60)	7 4 (57)	10 4 (40)
DC	7 0 (0)	5 3 (60)	5 3 (60)
PolyC	7 2 (29)	5 2 (40)	7 4 (57)
pM0	38 14 (37)	3 21 (64)	36 20 (56)
TC	25 12 (48)	2 17 (77)	25 15 (60)
DC	6 1 (17)	4 2 (50)	4 4 (100)
PolyC	7 1 (14)	7 2 (29)	7 1 (14)
pM1	16 9 (56)	12 8 (67)	16 8 (50)
TC	9 7 (78)	8 6 (75)	9 5 (56)
DC	4 0 (0)	3 2 (67)	4 1 (25)
PolyC	3 2 (67)	1 0 (0)	3 2 (67)

(a) Determination according to the newly revised classification of the AJCC and UICC. (b) TC: Intestinal or tubular carcinoma; DC: Diffuse carcinoma; PolyC: Mixed polymorphous carcinoma. (c) iNOS: Inducible nitric oxide synthase; NTYR: Nitrotyrosine; 8OH-dG: 8-hydroxy-2’-deoxyguanosine

Table 3 Correlation of staining with stage grouping^a

Stagesb	iNOScNo. of casesTotal positive(%)	NTYRcNo. of casesTotal positive (%)	8OH-dGcNo. of casesTotal positive (%)
Stage 0	7 1 (14)	7 6 (86)	10 5 (50)
Stage I	22 9 (41)	21 15 (71)	21 12 (57)
TC	16 8 (50)	16 12 (75)	16 10 (63)
DC	3 0 (0)	2 2 (100)	2 2 (100)
PolyC	3 1 (33)	3 1 (33)	3 0 (0)
Stage II+III	13 5 (38)	10 4 (40)	12 5 (42)
TC	8 4 (50)	5 4 (80)	7 3 (43)
DC	2 1 (50)	2 0 (0)	2 2 (100)
PolyC	3 0 (0)	3 0 (0)	3 0 (0)
Stage IV	19 9 (47)	14 10 (71)	18 10 (56)
TC	9 7 (78)	8 6 (75)	9 5 (56)
DC	5 0 (0)	3 2 (67)	4 1 (25)
PolyC	5 2 (40)	3 2 (67)	5 4 (80)
All stages (early cancer excluded)	36 16 (44)	29 17 (59)	35 17 (49)
TC	20 12 (60)	17 13 (76)	20 10 (50)
DC	7 1 (14)	5 2 (40)	6 3 (50)
DC+PolyC	16 4 (25)	12 4 (33)	15 7 (47)
Early cancer (pTis + pT1)	25 8 (32)	23 18 (78)	26 15 (58)
TC	20 8 (40)	19 15 (79)	22 13 (59)
DC	3 0 (0)	2 2 (100)	2 2 (100)
DC+PolyC	5 0 (0)	4 3 (75)	4 2 (50)

(a) Determination according to the newly revised classification of the AJCC and UICC. (b) TC: Intestinal or tubular carcinoma; DC: Diffuse carcinoma; PolyC: Mixed polymorphous carcinoma. (c) iNOS: Inducible nitric oxide synthase; NTYR: Nitrotyrosine; 8OH-dG: 8-hydroxy-2’-deoxyguanosine.

Table 4 Co-occurrence of immunostaining for iNOS, NTYR and 8-OH-dG^a

Markers	No. of samples (%)
	TCbn = 40	DCbn  = 12	PolyCbn  = 10
All negative	5 (12)	2 (16)	5 (50)
One positive
iNOS	2 (5)	1 (8)	1 (10)
NTYR	4 (10)	2 (17)	1 (10)
8-OHdG	2 (5)	2 (17)	1 (10)
Two positive
iNOS + NTYR	8 (20)	1 (8)	0 (0)
NTYR + 8-OHdG	8 (20)	3 (25)	2 (20)
iNOS + 8-OHdG	1 (2.5)	1 (8)	0 (0)
All positive
iNOS + NTYR + 8-OHdG	10 (25)	0 (0)	0 (0)

(a) iNOS: Inducible nitric oxide synthase; NTYR: Nitrotyrosine; 8OH-dG: 8-hydroxy-2’-deoxyguanosine. (b) TC: Intestinal or tubular carcinoma; DC: Diffuse carcinoma; PolyC: Mixed polymorphous carcinoma.