Current status and prospects of clinical proteomics studies on detection of colorectal cancer: Hopes and fears

Table 1 Recommendations of various pre-analytical variations from three MALDI-TOF based reproducibility studies

	Blood component	Peptide isolation	Temperature before sample handling (°C)	Time before centrifugation	Storage of serum	Freeze/thaw cycles	Circadian rhythm effect
Baumann et al	Serum plasma	C3, C8, C18 beads	21	< 30 min	-80 °C	1	NA1
de Noo et al	Serum	C8 beads	21	Ideally < 30 min, practically < 2-4 h	NA1	2	No effect
West-Nielsen et al	Serum plasma	C8 beads	21	< 8 h	-20/-80°C	1	NA1

¹Not applicable for this study.