Correlation of p53 gene mutation and expression of P53 protein in cholangiocarcinoma

Table 1 Primers sequences of p53 gene exons 5-8

Primers	Sequences
	Sense	Anti-sense
Exon 5	5′-TGT TCA CTT GTG CCC TGA CT-3′	5′-CAG CCC TGT CGT CTC TCC AG-3′
Exon 6	5′-ACA GGG CTG GTT GCC CAG GGT-3′	5′-CTC CCAGAG ACC CCA GTT GC-3′
Exon 7	5′-GGT CTC CCC AAG GCG CAC TGG-3′	5′-AGG CTG GGG GCA CAG CAG GCC-3′
Exon 8	5′-ATT TCC TTA CTG CCT CTT GC-3′	5′-AAG TGA ATC TGA GGC ATA AC-3

Table 2 Correlation of mutation status of p53 gene and P53 protein expression with pathologic parameters in cholangiocarcinoma

Pathologiccharacter	n	p53 gene mutation		P53 protein expression
		Positive (n, %)	P vaule	Positive (n, %)	P vaule
Pathologic type
Tubular	18	11 (61.1)		10 (55.6)
carcinoma
Papillary	9	6 (66.7)		5 ( 55.6)
carcinoma
Mucoid	4	2 (50.0)		2 (50.0)
carcinoma
Non-differentiated	5	3 (60.0)	> 0.05	2 (40.0)	> 0.05
carcinoma
Differentiation
Well -Moderate	25	13 (52.0)		9 (36.0)
Poor	11	9 (81.8)	> 0.05	10 (90.9)	< 0.05a
Invasion
T1	16	7 (43.8)		2 (12.5)
T2	10	8 (80.0)		8 (80.0)
T3	10	7 (70.0)	> 0.05	9 (90.0)	< 0.05c
Lymph node metastasis
N0	3	2 (66.7)		0 (0)
N1	33	20 (60.6)	> 0.05	19 (57.5)	> 0.05