Relation of overexpression of S phase kinase-associated protein 2 with reduced expression of p27 and PTEN in human gastric carcinoma

Table 1 Skp2 protein expression in human gastric tissues

frequency(%)	Specimens	Skp2 labeling frequency(%)
Tissue	(n)	Median	Mean±SE
Normal gastric mucosa	5	0.001	0.47±0.19
Chronic superficial gastritis	10	0.002	0.53±0.13
Intestinal metaplasia	30	12.003	12.68±0.86
Dysplasia	30	19.004	16.74±0.82
Primary gastric carcinoma	138	29.005	31.34±2.17
Adjacent mucosa	138	16.006	19.32±1.22
Metastasis tumor tissue in lymph node	102	39.507	39.76±2.00

^{1,2 ,3 ,4}Frequency was significantly higher than in normal gastric tissue and chronic superficial gastritis (P = 0.000,all the same; Mann-Whitney U test). ²Frequency was significantly higher than in intestinal metaplasia (P = 0.000; Mann-Whitney U test). ³Frequency was significantly higher than in intestinal metaplasia and dysplasia, (P = 0.000,all the same; Mann-Whitney U test). ⁵Frequency was Significantly higher than in primary tumors (P = 0.037; Mann-Whitney U test).

Table 2 Skp2 protein expression in 138 human gastric cancers

Clinicopathological feature	Specimens	Skp2 labeling frequency (%)
	(n)	Mean±SE	rho	P
Patients	138	36.32±1.22
Age (yr)	138	58.50	0.190	0.833
＜60	66	34.25±3.58
≥60	72	34.41±2.63
Gender			0.119	0.179
Females	27	24.96±5.21
Males	111	32.90±2.37
Differentiation grade1			0.315	0.000
Well/Moderate	63	22.41±2.59
Poor	75	38.85±3.10
Depth of invasion			0.001	0.986
Serosal	24	25.84±2.45
Outside soft tissue	114	29.25±4.87
Vessel invasion2			0.303	0.000
Absent	120	27.89±2.11
Present	18	54.39±6.84
Lymph node metastasis3			0.254	0.000
Absent	36	27.94±3.38
Present	102	37.14±2.69
Distant metastasis			0.091	0.307
Absent	26	22.77±6.92
Present	12	31.79±2.38
Clinical stage			0.069	0.440
0	0
Ia	0
Ib	3	22.78±6.92
II	36	29.81±3.14
IIIa	12	44.67±6.78
IIIb	66	34.50±3.55
IV	9	53.00±0.57

^{1, 2, 3}Skp2 labeling frequency was positively correlated with differentiated degree (rho = 0.315, P = 0.000), vessel invasion (rho = 0.303, P = 0.000), and lymph node metastasis (rho = 0.254, P = 0.000) in gastric carcinoma, respectively. ⁴Because clinical staging could not be performed due to the lack of accurate records about lymph node in operation, data of 12 cases were cancelled in statistical analysis.