Effect of SNC19/ST14 gene overexpression on invasion of colorectal cancer cells

Table 1 Percentage of cell adhesion to ECM (mean±SD)

Cell type	1 h	2 h	6 h	12 h
RKO	12.0 ± 1.8	52.7 ± 6.5	59.7 ± 2.9	98.5 ± 0.6
RKO-pSecTag	7.6 ± 1.8	42.8 ± 1.6	56.5 ± 3.4	93.4 ± 0.6
RKO-ST14-1	5.1 ± 1.9a	32.3 ± 0.8a	55.6 ± 5.7	88.5 ± 2.0

^aP<0.05 vs RKO and RKO-pSecTag.

Table 2 In vitro cell movement assay (cm, mean±SD)

Cell	24 h	48 h	P
R-P-media	0.56 ± 0.24	0.44 ± 0.08	0.22
R-S-media	0.73 ± 0.56	0.72 ± 0.45	0.15
R-P-serum	0.97 ± 0.07	1.01 ± 0.27	0.37
R-S-serum	1.15 ± 0.46	1.11 ± 0.6	0.7

Table 3 In vitro cell invasion assay (n = 30, mean±SD)

Cell line	Cell number/HP	P
RKO	32.6 ± 4.1	0.000018
RKO-pSecTag	44.9 ± 7.0	0.00009
RKO-ST14-1	64.5 ± 7.3