Effect of Helicobacter pylori infection on p53 expression of gastric mucosa and adenocarcinoma with microsatellite instability

Table 1 Primers of microsatellite markers

Markers	Primers	Tm (°C)
BAT-26	5-FAM- TGACTACTTTTGACTTCAGCC	58
	5-AACCATTCAACATTTTTAACCC
D17S261	5-HEX-AGGGATACTATTCAGCCCGAGGTG	60
	5-ACTGCCACTCCTTGCCCCATTC
D3S1283	5-TET-GGCAGTACCACCTGTAGAAATG	60
	5-GAGTAACAGAGGCATCGTGTATTC
D2S123	5-FAM-AAACAGGATGCCTGCCTTTA	60
	5'-GGACTTTCCACCTATGGGAC
D3S1611	5-HEX-CCCCAAGGCTGCACTT	60
	5-AGCTGAGACTACAGGCATTTG

Table 2 H pylori infection in the normal, gastritis, intestinal metaplasia, and tumor samples

Tissue type	Number of samples	Infection number of H pylori	Infection rates (%)
Normal	33	0	0
Gastritis	45	38	84.4
Intestinal metaplasia	30	23	76.7
Carcinoma	46	30	65.2

Table 3 MSI frequency according to H pylori status

MSI frequency	H pylori positive (30)	H pylori negative (16)	P1
MSI-H (12)	36.7% (11/30)	6.3% (1/16)	0.035
MSI-L (3)	6.7% (2/30)	6.25 % (1/16)	1.000

¹H pylori positive vs H pylori negative.

Table 4 P53 expression with regard to H pylori status in carcinomas

H pylori status	P53 positive (20)	P
H pylori positive (30)	17	0.0131
H pylori negative (16)	3

¹H pylori positive vs H pylori negative.

Table 5 Length of representative fragments of five microsatellite loci in the normal tissue and peripheral blood sample of the gastric cancer patient

	BAT-26	D17S261		D3S1283		D2S123	D3S1611
Peak no.	1# (bp)	2# (bp)	3# (bp)	4# (bp)	5# (bp)	6# (bp)	7# (bp)
Normal	119.15	127.52	133.78	149.12	147.25	209.42	262.76
Tumor	120.30	121.63	131.45	143.23	147.36	209.30	262.85