Possible mechanism for hepatitis B virus X gene to induce apoptosis of hepatocytes

Table 1 Primer sequences for PCR and amplification conditions for each target gene

Primer	Sequence	Amplification conditions	Product (base)
Fas	5-TCAGTACGGAGTTGGGGAAG-3’	Denaturation at 94 °C for 45 s,	207
	5-CAGGCCTTCCAAGTTCTGAG-3	annealing at 63 °C for 30 s and synthesizing
		at 72 °C for 1 min for 35 cycles
FasL	5-GATGATGGAGGGGAAGATGA-3	Denaturation at 94 °C for 45 s,	203
	5-TGGAAAGAATCCCAAAGTGC-3’	annealing at 58 °C for 30 s and synthesizing
		at 72 °C for 1 min for 30 cycles
Bax	5-TTTGCTTCAGGGTTTCATCC-3’	Denaturation at 94 °C for 45 s,	246
	5-CAGTTGAAGTTGCCGTCAGA-3’	annealing at 58 °C for 30 s and synthesizing
		at 72 °C for 1 min for 30 cycles
Bcl-2	5-CGACGACTTCTCCCGCCGCTACCGC-3’	Denaturation at 94 °C for 45 s,	318
	5-CCGCATGCTGGGGCCGTACAGTTCC-3’	annealing at 67 °C for 30 s and synthesizing
		at 72 °C for 1 min for 30 cycles
c-myc	5-TTCGGGTAGTGGAAAACCAG-3’	Denaturation at 94 °C for 45 s,	203
	5-CAGCAGCTCGAATTTCTTCC-3’	annealing at 58 °C for 30 s and synthesizing
		at 72 °C for 1 min for 28 cycles
GAPDH	5-ACCACAGTCCATGCCATCAC-3’	Changed according to different target genes	452
	5-TCCACCACCCTGTTGCTGTA-3’