Apoptosis and its pathway in X gene-transfected HepG2 cells

Table 1 Sequences of gene primers and amplification conditions

Target gene	Primer sequences	Amplification conditions	Product (base)
Fas	5-TCA GTA CGG AGT TGG GGA AG-3’	Denaturation at 94 °C for 45 s, annealing	207 bp
	5-CAG GCC TTC CAA GTT CTG AG-3’	at 64 °C for 30 s, extension at 72 °C for
		1 min , 35 cycles
FasL	5-GAT GAT GGA GGG GAA GAT GA-3’	Denaturation at 94 °C for 45 s,	203 bp
	5-TGG AAA GAA TCC CAA AGT GC-3’	annealing at 58 °C for 30 s, extension at
		72 °C for l min, 38 cycles
Bax	5-TTT GCT TCA GGG TTT CAT CC-3’	Denaturation at 94 °C for 45 s, annealing at	246 bp
	5-CAG TTG AAG TTG CCG TCA GA-3’	58 °C for 30 s, extension at 72 °C for
		1 min, 30 cycles
Bcl-xL	5-GGC TGG GAT ACT TTT GTG GA-3’	Denaturation at 94 °C for 45 s, annealing at	198 bp
	5-ATG TGG TGG AGC AGA GAA GG-3’	64 °C for 30 s, extension at 72 °C for
		45 s, 25 cycles
c-myc	5-TTC GGG TAG TGG AAA ACC AG-3’	Denaturation at 94 °C for 45 s, annealing at	203 bp
	5-CAG CAG CTC GAA TTT CTT CC-3’	58 °C for 30 s, extension at 72 °C for
		1 min, 30 cycles
β-actin	5-GGC ATC GTG ATG GAC TCC G-3’	Changed according to different target genes	607 bp
	5-GCT GGA AGG TGG ACA GCG A-3’

Table 2 A₄₉₂ of HepG₂, HepG₂/pcDNA3 and HepG₂/pcDNA3-X cells (mean±SE)

Group	n	A492
HepG2	10	0.14410±0.004927
HepG2/pcDNA3	10	0.12150±0.007159
HepG2/pcDNA3-X	10	0.08910±0.003164

Table 3 Effect of HBx on cell apoptosis of HepG₂ cells (mean±SE)

Group	n	Apoptosis
HepG2	5	0.2200±0.1000
HepG2/pcDNA3	5	0.2800±0.1000
HepG2/pcDNA3-X	5	0.4750±0.015